Evaluation of anti-cancer drug Suberoylanilide Hydroxamic Acid (SAHA) on cancer cell phenotype in a three-dimensional (3D) breast tumor-stroma platform

N. Peela, D. Truong, E. S. Barrientos, Ghassan Mouneimne, M. Nikkhah

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We report a microfluidic platform with distinct tumor and stromal regions for preclinical anticancer drug evaluation. Highly metastatic SUM159 breast cancer cells were encapsulated in 3D matrix and injected into the tumor region of the microfluidic platform. The cells adopted an invasive morphology and proliferated profusely, migrating from the tumor compartment to the stromal region. Addition of FDA-approved histone deacetylase inhibitor (HDACi) Suberoylanilide Hydroxamic Acid (SAHA) into the cell media substantially inhibited migration of cancer cells, inducing morphological changes such as elongation and increased protrusions. The proposed platform enables studies on anticancer drug interactions within a physiologically relevant tumor-stroma microenvironment.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages565-566
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Chemotherapeutic
  • Microfluidic
  • Tumor microenvironment
  • Vorinostat (SAHA)

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Peela, N., Truong, D., Barrientos, E. S., Mouneimne, G., & Nikkhah, M. (2016). Evaluation of anti-cancer drug Suberoylanilide Hydroxamic Acid (SAHA) on cancer cell phenotype in a three-dimensional (3D) breast tumor-stroma platform. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 565-566). Chemical and Biological Microsystems Society.