Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress

L. W. Hall, F. R. Dunshea, J. D. Allen, S. Rungruang, J. L. Collier, N. M. Long, Robert J Collier

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Betaine (BET), a natural, organic osmolyte, improves cellular efficiency by acting as a chaperone, refolding denatured proteins. To test if dietary BET reduced the effect of heat stress (HS) in lactating dairy cows, multiparous, lactating Holstein cows (n = 24) were blocked by days in milk (101.4 ± 8.6 d) and randomly assigned to 1 of 3 daily intakes of dietary BET: the control (CON) group received no BET, mid intake (MID) received 57 mg of BET/kg of body weight, and high dose (HI) received 114 mg of BET/kg of body weight. Cows were fed twice daily and BET was top-dressed at each feeding. Cows were milked 2 times/d and milk samples were taken daily for analysis. Milk components, yield, feed intake, and water intake records were taken daily. Rectal temperature and respiration rate were taken 3 times/d at 0600, 1400, and 1800 h. Cows were housed in environmentally controlled rooms and were allowed acclimation for 7 d at thermoneutral (TN) conditions with a mean temperature-humidity index of 56.6. Cows were then exposed to 7 d of TN followed by 7 d of HS represented by a temperature-humidity index of 71.5 for 14 d. This was followed by a recovery period of 3 d at TN. Dietary BET increased milk yield during the TN period. No differences were found between BET and CON in total milk production or milk composition during HS. The increase in water intake during HS was not as great for cows fed BET compared with controls. The cows on CON diets had higher p.m. respiration rate than both MID and HI BET during HS, but lower rectal temperature compared with BET. No difference was found in serum glucose during TN, but cows given HI had elevated glucose levels during HS compared with CON. No differences were found in serum insulin levels between CON and BET but an intake by environment interaction was present with insulin increasing in HI-treated lactating dairy cows during HS. The heat shock response [heat shock protein (HSP) 27 and HSP70] was upregulated in bovine mammary epithelial cells in vitro. Blood leukocyte HSP27 was downregulated at the HI dose under TN conditions and HSP70 was upregulated at the HI dose and this effect was increased by HS. No effect was seen with the MID dose with HSP27 or HSP70. The lack of effect of BET at MID may be associated with uptake across the gut. We conclude that BET increased milk production under TN conditions and was associated with reduced feed and water intake and slightly increased body temperatures during HS of cows fed BET. The effect of BET on milk production was lost during HS with HI BET, whereas serum glucose levels increased during HS.

Original languageEnglish (US)
Pages (from-to)9745-9753
Number of pages9
JournalJournal of Dairy Science
Volume99
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Betaine
betaine
heat stress
Holstein
Hot Temperature
cows
Milk
blood serum
Drinking
milk production
Temperature
Respiratory Rate
dosage
respiratory rate
Humidity
milk composition
Glucose
glucose
humidity
temperature

Keywords

  • betaine
  • blood glucose
  • dairy cow
  • heat stress

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Hall, L. W., Dunshea, F. R., Allen, J. D., Rungruang, S., Collier, J. L., Long, N. M., & Collier, R. J. (2016). Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. Journal of Dairy Science, 99(12), 9745-9753. https://doi.org/10.3168/jds.2015-10514

Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. / Hall, L. W.; Dunshea, F. R.; Allen, J. D.; Rungruang, S.; Collier, J. L.; Long, N. M.; Collier, Robert J.

In: Journal of Dairy Science, Vol. 99, No. 12, 01.12.2016, p. 9745-9753.

Research output: Contribution to journalArticle

Hall, LW, Dunshea, FR, Allen, JD, Rungruang, S, Collier, JL, Long, NM & Collier, RJ 2016, 'Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress', Journal of Dairy Science, vol. 99, no. 12, pp. 9745-9753. https://doi.org/10.3168/jds.2015-10514
Hall LW, Dunshea FR, Allen JD, Rungruang S, Collier JL, Long NM et al. Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. Journal of Dairy Science. 2016 Dec 1;99(12):9745-9753. https://doi.org/10.3168/jds.2015-10514
Hall, L. W. ; Dunshea, F. R. ; Allen, J. D. ; Rungruang, S. ; Collier, J. L. ; Long, N. M. ; Collier, Robert J. / Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. In: Journal of Dairy Science. 2016 ; Vol. 99, No. 12. pp. 9745-9753.
@article{383b37fa6294417f84093bd09a1ea062,
title = "Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress",
abstract = "Betaine (BET), a natural, organic osmolyte, improves cellular efficiency by acting as a chaperone, refolding denatured proteins. To test if dietary BET reduced the effect of heat stress (HS) in lactating dairy cows, multiparous, lactating Holstein cows (n = 24) were blocked by days in milk (101.4 ± 8.6 d) and randomly assigned to 1 of 3 daily intakes of dietary BET: the control (CON) group received no BET, mid intake (MID) received 57 mg of BET/kg of body weight, and high dose (HI) received 114 mg of BET/kg of body weight. Cows were fed twice daily and BET was top-dressed at each feeding. Cows were milked 2 times/d and milk samples were taken daily for analysis. Milk components, yield, feed intake, and water intake records were taken daily. Rectal temperature and respiration rate were taken 3 times/d at 0600, 1400, and 1800 h. Cows were housed in environmentally controlled rooms and were allowed acclimation for 7 d at thermoneutral (TN) conditions with a mean temperature-humidity index of 56.6. Cows were then exposed to 7 d of TN followed by 7 d of HS represented by a temperature-humidity index of 71.5 for 14 d. This was followed by a recovery period of 3 d at TN. Dietary BET increased milk yield during the TN period. No differences were found between BET and CON in total milk production or milk composition during HS. The increase in water intake during HS was not as great for cows fed BET compared with controls. The cows on CON diets had higher p.m. respiration rate than both MID and HI BET during HS, but lower rectal temperature compared with BET. No difference was found in serum glucose during TN, but cows given HI had elevated glucose levels during HS compared with CON. No differences were found in serum insulin levels between CON and BET but an intake by environment interaction was present with insulin increasing in HI-treated lactating dairy cows during HS. The heat shock response [heat shock protein (HSP) 27 and HSP70] was upregulated in bovine mammary epithelial cells in vitro. Blood leukocyte HSP27 was downregulated at the HI dose under TN conditions and HSP70 was upregulated at the HI dose and this effect was increased by HS. No effect was seen with the MID dose with HSP27 or HSP70. The lack of effect of BET at MID may be associated with uptake across the gut. We conclude that BET increased milk production under TN conditions and was associated with reduced feed and water intake and slightly increased body temperatures during HS of cows fed BET. The effect of BET on milk production was lost during HS with HI BET, whereas serum glucose levels increased during HS.",
keywords = "betaine, blood glucose, dairy cow, heat stress",
author = "Hall, {L. W.} and Dunshea, {F. R.} and Allen, {J. D.} and S. Rungruang and Collier, {J. L.} and Long, {N. M.} and Collier, {Robert J}",
year = "2016",
month = "12",
day = "1",
doi = "10.3168/jds.2015-10514",
language = "English (US)",
volume = "99",
pages = "9745--9753",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress

AU - Hall, L. W.

AU - Dunshea, F. R.

AU - Allen, J. D.

AU - Rungruang, S.

AU - Collier, J. L.

AU - Long, N. M.

AU - Collier, Robert J

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Betaine (BET), a natural, organic osmolyte, improves cellular efficiency by acting as a chaperone, refolding denatured proteins. To test if dietary BET reduced the effect of heat stress (HS) in lactating dairy cows, multiparous, lactating Holstein cows (n = 24) were blocked by days in milk (101.4 ± 8.6 d) and randomly assigned to 1 of 3 daily intakes of dietary BET: the control (CON) group received no BET, mid intake (MID) received 57 mg of BET/kg of body weight, and high dose (HI) received 114 mg of BET/kg of body weight. Cows were fed twice daily and BET was top-dressed at each feeding. Cows were milked 2 times/d and milk samples were taken daily for analysis. Milk components, yield, feed intake, and water intake records were taken daily. Rectal temperature and respiration rate were taken 3 times/d at 0600, 1400, and 1800 h. Cows were housed in environmentally controlled rooms and were allowed acclimation for 7 d at thermoneutral (TN) conditions with a mean temperature-humidity index of 56.6. Cows were then exposed to 7 d of TN followed by 7 d of HS represented by a temperature-humidity index of 71.5 for 14 d. This was followed by a recovery period of 3 d at TN. Dietary BET increased milk yield during the TN period. No differences were found between BET and CON in total milk production or milk composition during HS. The increase in water intake during HS was not as great for cows fed BET compared with controls. The cows on CON diets had higher p.m. respiration rate than both MID and HI BET during HS, but lower rectal temperature compared with BET. No difference was found in serum glucose during TN, but cows given HI had elevated glucose levels during HS compared with CON. No differences were found in serum insulin levels between CON and BET but an intake by environment interaction was present with insulin increasing in HI-treated lactating dairy cows during HS. The heat shock response [heat shock protein (HSP) 27 and HSP70] was upregulated in bovine mammary epithelial cells in vitro. Blood leukocyte HSP27 was downregulated at the HI dose under TN conditions and HSP70 was upregulated at the HI dose and this effect was increased by HS. No effect was seen with the MID dose with HSP27 or HSP70. The lack of effect of BET at MID may be associated with uptake across the gut. We conclude that BET increased milk production under TN conditions and was associated with reduced feed and water intake and slightly increased body temperatures during HS of cows fed BET. The effect of BET on milk production was lost during HS with HI BET, whereas serum glucose levels increased during HS.

AB - Betaine (BET), a natural, organic osmolyte, improves cellular efficiency by acting as a chaperone, refolding denatured proteins. To test if dietary BET reduced the effect of heat stress (HS) in lactating dairy cows, multiparous, lactating Holstein cows (n = 24) were blocked by days in milk (101.4 ± 8.6 d) and randomly assigned to 1 of 3 daily intakes of dietary BET: the control (CON) group received no BET, mid intake (MID) received 57 mg of BET/kg of body weight, and high dose (HI) received 114 mg of BET/kg of body weight. Cows were fed twice daily and BET was top-dressed at each feeding. Cows were milked 2 times/d and milk samples were taken daily for analysis. Milk components, yield, feed intake, and water intake records were taken daily. Rectal temperature and respiration rate were taken 3 times/d at 0600, 1400, and 1800 h. Cows were housed in environmentally controlled rooms and were allowed acclimation for 7 d at thermoneutral (TN) conditions with a mean temperature-humidity index of 56.6. Cows were then exposed to 7 d of TN followed by 7 d of HS represented by a temperature-humidity index of 71.5 for 14 d. This was followed by a recovery period of 3 d at TN. Dietary BET increased milk yield during the TN period. No differences were found between BET and CON in total milk production or milk composition during HS. The increase in water intake during HS was not as great for cows fed BET compared with controls. The cows on CON diets had higher p.m. respiration rate than both MID and HI BET during HS, but lower rectal temperature compared with BET. No difference was found in serum glucose during TN, but cows given HI had elevated glucose levels during HS compared with CON. No differences were found in serum insulin levels between CON and BET but an intake by environment interaction was present with insulin increasing in HI-treated lactating dairy cows during HS. The heat shock response [heat shock protein (HSP) 27 and HSP70] was upregulated in bovine mammary epithelial cells in vitro. Blood leukocyte HSP27 was downregulated at the HI dose under TN conditions and HSP70 was upregulated at the HI dose and this effect was increased by HS. No effect was seen with the MID dose with HSP27 or HSP70. The lack of effect of BET at MID may be associated with uptake across the gut. We conclude that BET increased milk production under TN conditions and was associated with reduced feed and water intake and slightly increased body temperatures during HS of cows fed BET. The effect of BET on milk production was lost during HS with HI BET, whereas serum glucose levels increased during HS.

KW - betaine

KW - blood glucose

KW - dairy cow

KW - heat stress

UR - http://www.scopus.com/inward/record.url?scp=84992135655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992135655&partnerID=8YFLogxK

U2 - 10.3168/jds.2015-10514

DO - 10.3168/jds.2015-10514

M3 - Article

C2 - 27720159

AN - SCOPUS:84992135655

VL - 99

SP - 9745

EP - 9753

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 12

ER -