Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay

Adam Orendain, Jose Carrasco, Eniko T Enikov, Gholam Peyman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Central retinal vein occlusion (CRVO) is a vascular disease characterized by thrombosis of the retinal veins that can eventually lead to ischemia. Ischemic CRVO can then cause macular degeneration and neovascular glaucoma causing partial to full blindness. In this study, we determined the feasibility of electrospinning tubular scaffolds for treating CRVO and vascular disease. Electrospinning was utilized to produce customizable scaffolds from nano-bers using collagen type I. Scaffolds were treated with glutaraldehyde, glycine, ethanol, UV light, and combinations of the treatments for the purpose cross-linking and to study their angiogenic effects. Structural properties of the scaffolds were analyzed with scanning electron micrsoscopy (SEM). Scaffolds were immobilized with human recombinant vascular endothelial growth factor (rhVEGF165) to investigate the drugdelivering abilities of the electrospun materials and as a method to produce vascularization. The chick chorioallantoic membrane (CAM) assay was used to examine the effects of VEGF immobilizations and to evaluate the feasibility of creating an anastomosis to treat CRVO. Collagen onplants (non-electrospun) and electrospun implants were made on day 10 of embryonic development. Findings show collagen loaded with rhVEGF165 had improved vasculature and pro-angiogenic properties. The present study suggests that collagen can immobilize and release growth factor, be electrospun to mimic the ultrastructure of native blood vessels, and holds promise for vascular tissue engineering.

Original languageEnglish (US)
Title of host publicationASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
PublisherAmerican Society of Mechanical Engineers (ASME)
Volume3 B
ISBN (Print)9780791856222
DOIs
StatePublished - 2013
EventASME 2013 International Mechanical Engineering Congress and Exposition, IMECE 2013 - San Diego, CA, United States
Duration: Nov 15 2013Nov 21 2013

Other

OtherASME 2013 International Mechanical Engineering Congress and Exposition, IMECE 2013
CountryUnited States
CitySan Diego, CA
Period11/15/1311/21/13

Fingerprint

Cams
Collagen
Scaffolds
Assays
Electrospinning
Scaffolds (biology)
Blood vessels
Tissue engineering
Ultraviolet radiation
Amino acids
Structural properties
Ethanol
Membranes
Scanning
Electrons
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Orendain, A., Carrasco, J., Enikov, E. T., & Peyman, G. (2013). Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay. In ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE) (Vol. 3 B). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2013-64687

Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay. / Orendain, Adam; Carrasco, Jose; Enikov, Eniko T; Peyman, Gholam.

ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE). Vol. 3 B American Society of Mechanical Engineers (ASME), 2013.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Orendain, A, Carrasco, J, Enikov, ET & Peyman, G 2013, Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay. in ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE). vol. 3 B, American Society of Mechanical Engineers (ASME), ASME 2013 International Mechanical Engineering Congress and Exposition, IMECE 2013, San Diego, CA, United States, 11/15/13. https://doi.org/10.1115/IMECE2013-64687
Orendain A, Carrasco J, Enikov ET, Peyman G. Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay. In ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE). Vol. 3 B. American Society of Mechanical Engineers (ASME). 2013 https://doi.org/10.1115/IMECE2013-64687
Orendain, Adam ; Carrasco, Jose ; Enikov, Eniko T ; Peyman, Gholam. / Evaluation of electro-spun tubular scaffolds to create an anastomosis using the cam assay. ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE). Vol. 3 B American Society of Mechanical Engineers (ASME), 2013.
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