Evaluation of the deoxyuridine suppression test by using whole blood samples from folic acid-supplemented subjects

Tsunenobu Tamura, S. J. Soong, Howerde E. Sauberlich, Kenneth D Hatch, Philip Cole, C. E. Butterworth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We performed the deoxyuridine suppression test (dUST) along with assessment of folate and vitamin B-12 status in blood specimens from 136 normal women and 109 women with cervical dysplasia. All tests were repeated at 2,4, and 6 mo in subjects with dysplasia during a randomized, double-blind intervention trial in which 50 received a 10-mg daily oral folic acid supplement (F group) and 59 received a placebo (P group). Median folate concentration increased fivefold in plasma and threefold in erythrocytes of F group beginning at the second month and remained elevated whereas concentrations of the P group remained unchanged. Vitamin B-12 values did not vary significantly in either group. The dUST value decreased from 10.4 ± 4.6% (x̄ ± SD) pretreatment to 4.5 ± 4.7% in F group after 2 mo (p < 0.001). The dUST values had significant negative correlation with plasma and erythrocyte folate concentrations. However, erythrocyte folate had the greatest power to distinguish P group from F group.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume51
Issue number1
StatePublished - Jan 1990
Externally publishedYes

Fingerprint

Deoxyuridine
Folic Acid
folic acid
blood
erythrocytes
Erythrocytes
vitamin B12
Vitamin B 12
testing
sampling
Uterine Cervical Dysplasia
placebos
mouth
pretreatment
Placebos

Keywords

  • Cervical dysplasia
  • Deoxyuridine suppression test
  • Folate
  • Folic acid
  • Vitamin B-12

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Evaluation of the deoxyuridine suppression test by using whole blood samples from folic acid-supplemented subjects. / Tamura, Tsunenobu; Soong, S. J.; Sauberlich, Howerde E.; Hatch, Kenneth D; Cole, Philip; Butterworth, C. E.

In: American Journal of Clinical Nutrition, Vol. 51, No. 1, 01.1990, p. 80-86.

Research output: Contribution to journalArticle

Tamura, Tsunenobu ; Soong, S. J. ; Sauberlich, Howerde E. ; Hatch, Kenneth D ; Cole, Philip ; Butterworth, C. E. / Evaluation of the deoxyuridine suppression test by using whole blood samples from folic acid-supplemented subjects. In: American Journal of Clinical Nutrition. 1990 ; Vol. 51, No. 1. pp. 80-86.
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