Evaluation of the deoxyuridine suppression test by using whole blood samples from folic acid-supplemented subjects

T. Tamura, S. J. Soong, H. E. Sauberlich, K. D. Hatch, P. Cole, C. E. Butterworth

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We performed the deoxyuridine suppression test (dUST) along with assessment of folate and vitamin B-12 status in blood specimens from 136 normal women and 109 women with cervical dysplasia. All tests were repeated at 2, 4, and 6 mo in subjects with dysplasia during a randomized, double-blind intervention trial in which 50 received a 10-mg daily oral folic acid supplement (F group) and 59 received a placebo (P group). Median folate concentration increased fivefold in plasma and threefold in erythrocytes of F group beginning at the second month and remained elevated whereas concentrations of the P group remained unchanged. Vitamin B-12 values did not vary significantly in either group. The dUST value decreased from 10.4 ± 4.6% (x̄ ± SD) pretreatment to 4.5 ± 4.7% in F group after 2 mo (p < 0.001). The dUST values had significant negative correlation with plasma and erythrocyte folate concentrations. However, erythrocyte folate had the greatest power to distinguish P group from F group.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume51
Issue number1
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Keywords

  • cervical dysplasia
  • deoxyuridine suppression test
  • folate
  • folic acid
  • vitamin B-12

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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