Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy

Pierre Lucas, David Le Coq, Christophe Juncker, Jayne Collier, Dianne E. Boesewetter, Catherine Boussard-Pledel, Bruno Bureau, Mark R. Riley

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for Infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-100, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800-3000 cm -1 in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of Ober and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane sohibilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalApplied Spectroscopy
Volume59
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Poisons
evanescent waves
lungs
Spectroscopy
Cell membranes
membranes
fibers
Fibers
evaluation
cells
spectroscopy
Membranes
lipids
Octoxynol
Membrane Lipids
Hydrocarbons
L-Lactate Dehydrogenase
Surface-Active Agents
Lipids
lactates

Keywords

  • Cell culture
  • Chalcogenide glass fiber
  • Fourier transform infrared spectroscopy
  • FT-IR spectroscopy
  • Human lung cells
  • Toxicity monitoring

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Lucas, P., Coq, D. L., Juncker, C., Collier, J., Boesewetter, D. E., Boussard-Pledel, C., ... Riley, M. R. (2005). Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy. Applied Spectroscopy, 59(1), 1-9. https://doi.org/10.1366/0003702052940387

Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy. / Lucas, Pierre; Coq, David Le; Juncker, Christophe; Collier, Jayne; Boesewetter, Dianne E.; Boussard-Pledel, Catherine; Bureau, Bruno; Riley, Mark R.

In: Applied Spectroscopy, Vol. 59, No. 1, 01.2005, p. 1-9.

Research output: Contribution to journalArticle

Lucas, P, Coq, DL, Juncker, C, Collier, J, Boesewetter, DE, Boussard-Pledel, C, Bureau, B & Riley, MR 2005, 'Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy', Applied Spectroscopy, vol. 59, no. 1, pp. 1-9. https://doi.org/10.1366/0003702052940387
Lucas, Pierre ; Coq, David Le ; Juncker, Christophe ; Collier, Jayne ; Boesewetter, Dianne E. ; Boussard-Pledel, Catherine ; Bureau, Bruno ; Riley, Mark R. / Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy. In: Applied Spectroscopy. 2005 ; Vol. 59, No. 1. pp. 1-9.
@article{f3e3782d20f0486188234e107646ae56,
title = "Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy",
abstract = "Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for Infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-100, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800-3000 cm -1 in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of Ober and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane sohibilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.",
keywords = "Cell culture, Chalcogenide glass fiber, Fourier transform infrared spectroscopy, FT-IR spectroscopy, Human lung cells, Toxicity monitoring",
author = "Pierre Lucas and Coq, {David Le} and Christophe Juncker and Jayne Collier and Boesewetter, {Dianne E.} and Catherine Boussard-Pledel and Bruno Bureau and Riley, {Mark R.}",
year = "2005",
month = "1",
doi = "10.1366/0003702052940387",
language = "English (US)",
volume = "59",
pages = "1--9",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "1",

}

TY - JOUR

T1 - Evaluation of toxic agent effects on lung cells by fiber evanescent wave spectroscopy

AU - Lucas, Pierre

AU - Coq, David Le

AU - Juncker, Christophe

AU - Collier, Jayne

AU - Boesewetter, Dianne E.

AU - Boussard-Pledel, Catherine

AU - Bureau, Bruno

AU - Riley, Mark R.

PY - 2005/1

Y1 - 2005/1

N2 - Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for Infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-100, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800-3000 cm -1 in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of Ober and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane sohibilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.

AB - Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for Infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-100, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800-3000 cm -1 in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of Ober and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane sohibilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.

KW - Cell culture

KW - Chalcogenide glass fiber

KW - Fourier transform infrared spectroscopy

KW - FT-IR spectroscopy

KW - Human lung cells

KW - Toxicity monitoring

UR - http://www.scopus.com/inward/record.url?scp=12344253803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12344253803&partnerID=8YFLogxK

U2 - 10.1366/0003702052940387

DO - 10.1366/0003702052940387

M3 - Article

C2 - 15720730

AN - SCOPUS:12344253803

VL - 59

SP - 1

EP - 9

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 1

ER -