Evanescent-imaging-ellipsometry-based microarray reader

Srivatsa Venkatasubbarao, Neil Beaudry, Yanming Zhao, Russell A Chipman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We describe the development of a label-less ellipsometric imaging microarray reader. The ability of the ellipsometric microarray reader to measure binding of sample to microarray surface is verified using oligonucleotide complementary DNA (cDNA) microarrays. Polarized light illuminates the microarray surface through a glass substrate at an angle beyond the critical angle and changes in the polarization of totally internally reflected light resulting from binding events on the microarray surface are measured. This polarization change is used to measure the thickness of biomolecules bound to the microarray. A prototype ellipsometric imaging microarray reader is constructed and calibrated, and the performance is evaluated with cDNA microarrays. The microarray reader measures changes in refractive index changes as small as 0.0024 and thickness changes as small as 0.28 nm. The optimization of angle of incidence and substrate refractive index necessary to achieve high sensitivity is also described. This ellipsometric technique offers an attractive alternative to fluorescence-microarray readers in some genomic, proteomic, diagnostic, and sensing applications.

Original languageEnglish (US)
Article number014028
JournalJournal of Biomedical Optics
Volume11
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Refractometry
Ellipsometry
readers
Microarrays
Oligonucleotide Array Sequence Analysis
ellipsometry
Complementary DNA
Imaging techniques
Light
complementary DNA
Oligonucleotides
Proteomics
Glass
Fluorescence
refractivity
Incidence
oligonucleotides
polarization
polarized light
incidence

Keywords

  • Diagnostics
  • Ellipsometry
  • Fluorescence
  • Label-less
  • Microarray

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Evanescent-imaging-ellipsometry-based microarray reader. / Venkatasubbarao, Srivatsa; Beaudry, Neil; Zhao, Yanming; Chipman, Russell A.

In: Journal of Biomedical Optics, Vol. 11, No. 1, 014028, 01.2006.

Research output: Contribution to journalArticle

Venkatasubbarao, Srivatsa ; Beaudry, Neil ; Zhao, Yanming ; Chipman, Russell A. / Evanescent-imaging-ellipsometry-based microarray reader. In: Journal of Biomedical Optics. 2006 ; Vol. 11, No. 1.
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