Evidence for a subpopulation of antigen-presenting cells specific for the induction of the delayed-type hypersensitivity response

Glenn K. Matsushima, Wendy Gilmore, Nancy Casteel, Jeffrey A Frelinger, Stephen A. Stohlman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Young adult SJL mice (8 weeks of age or younger) do not mount a delayed-type hypersensitivity (DTH) response due to the failure of a macrophage antigen-presenting cell (APC) to induce TDTH effector cells. SJL mice that are 10 weeks of age or older produce a normal DTH response. This genetic defect provides a model for the investigation of functional subpopulations of APC which interact with specific subpopulations of T cells. In this study, we used this model to examine whether macrophage APC impairment involves APC-dependent immune responses other than DTH. No age-dependent differences were found in the ability of spleen cells from SJL mice to proliferate and synthesize interleukin-2 in response to concanavalin A; nor was the proliferative response to a variety of antigenic stimuli affected. In addition, no differences were observed in the contact sensitivity response or in the in vitro generation of allogeneic cytotoxic T lymphocytes (CTL). In contrast, the in vivo generation of allogeneic CTL was significantly depressed in 6-week-old SJL and could not be restored to normal by the adoptive transfer of macrophages from DTH responsive 12-week-old SJL mice. Finally, examination of the humoral response of 6-week-old SJL indicated no impairment in IgM or IgG serum antibody levels or in the induction of splenic B cells. Thus, the macrophage APC regulating the induction of TDTH effector cells does not appear to participate in the induction of T helper cells for other cellular and humoral responses. These data support the hypothesis that distinct subpopulations of APC may regulate the induction of specific immune effector mechanisms.

Original languageEnglish (US)
Pages (from-to)171-181
Number of pages11
JournalCellular Immunology
Volume119
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Delayed Hypersensitivity
Antigen-Presenting Cells
Macrophages
Cytotoxic T-Lymphocytes
Adoptive Transfer
Contact Dermatitis
Concanavalin A
Helper-Inducer T-Lymphocytes
Interleukin-2
Immunoglobulin M
Young Adult
B-Lymphocytes
Spleen
Immunoglobulin G
T-Lymphocytes
Antibodies
Serum

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Evidence for a subpopulation of antigen-presenting cells specific for the induction of the delayed-type hypersensitivity response. / Matsushima, Glenn K.; Gilmore, Wendy; Casteel, Nancy; Frelinger, Jeffrey A; Stohlman, Stephen A.

In: Cellular Immunology, Vol. 119, No. 1, 1989, p. 171-181.

Research output: Contribution to journalArticle

Matsushima, Glenn K. ; Gilmore, Wendy ; Casteel, Nancy ; Frelinger, Jeffrey A ; Stohlman, Stephen A. / Evidence for a subpopulation of antigen-presenting cells specific for the induction of the delayed-type hypersensitivity response. In: Cellular Immunology. 1989 ; Vol. 119, No. 1. pp. 171-181.
@article{d62b3dea25034134aaf10fa62563ee61,
title = "Evidence for a subpopulation of antigen-presenting cells specific for the induction of the delayed-type hypersensitivity response",
abstract = "Young adult SJL mice (8 weeks of age or younger) do not mount a delayed-type hypersensitivity (DTH) response due to the failure of a macrophage antigen-presenting cell (APC) to induce TDTH effector cells. SJL mice that are 10 weeks of age or older produce a normal DTH response. This genetic defect provides a model for the investigation of functional subpopulations of APC which interact with specific subpopulations of T cells. In this study, we used this model to examine whether macrophage APC impairment involves APC-dependent immune responses other than DTH. No age-dependent differences were found in the ability of spleen cells from SJL mice to proliferate and synthesize interleukin-2 in response to concanavalin A; nor was the proliferative response to a variety of antigenic stimuli affected. In addition, no differences were observed in the contact sensitivity response or in the in vitro generation of allogeneic cytotoxic T lymphocytes (CTL). In contrast, the in vivo generation of allogeneic CTL was significantly depressed in 6-week-old SJL and could not be restored to normal by the adoptive transfer of macrophages from DTH responsive 12-week-old SJL mice. Finally, examination of the humoral response of 6-week-old SJL indicated no impairment in IgM or IgG serum antibody levels or in the induction of splenic B cells. Thus, the macrophage APC regulating the induction of TDTH effector cells does not appear to participate in the induction of T helper cells for other cellular and humoral responses. These data support the hypothesis that distinct subpopulations of APC may regulate the induction of specific immune effector mechanisms.",
author = "Matsushima, {Glenn K.} and Wendy Gilmore and Nancy Casteel and Frelinger, {Jeffrey A} and Stohlman, {Stephen A.}",
year = "1989",
doi = "10.1016/0008-8749(89)90233-5",
language = "English (US)",
volume = "119",
pages = "171--181",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence for a subpopulation of antigen-presenting cells specific for the induction of the delayed-type hypersensitivity response

AU - Matsushima, Glenn K.

AU - Gilmore, Wendy

AU - Casteel, Nancy

AU - Frelinger, Jeffrey A

AU - Stohlman, Stephen A.

PY - 1989

Y1 - 1989

N2 - Young adult SJL mice (8 weeks of age or younger) do not mount a delayed-type hypersensitivity (DTH) response due to the failure of a macrophage antigen-presenting cell (APC) to induce TDTH effector cells. SJL mice that are 10 weeks of age or older produce a normal DTH response. This genetic defect provides a model for the investigation of functional subpopulations of APC which interact with specific subpopulations of T cells. In this study, we used this model to examine whether macrophage APC impairment involves APC-dependent immune responses other than DTH. No age-dependent differences were found in the ability of spleen cells from SJL mice to proliferate and synthesize interleukin-2 in response to concanavalin A; nor was the proliferative response to a variety of antigenic stimuli affected. In addition, no differences were observed in the contact sensitivity response or in the in vitro generation of allogeneic cytotoxic T lymphocytes (CTL). In contrast, the in vivo generation of allogeneic CTL was significantly depressed in 6-week-old SJL and could not be restored to normal by the adoptive transfer of macrophages from DTH responsive 12-week-old SJL mice. Finally, examination of the humoral response of 6-week-old SJL indicated no impairment in IgM or IgG serum antibody levels or in the induction of splenic B cells. Thus, the macrophage APC regulating the induction of TDTH effector cells does not appear to participate in the induction of T helper cells for other cellular and humoral responses. These data support the hypothesis that distinct subpopulations of APC may regulate the induction of specific immune effector mechanisms.

AB - Young adult SJL mice (8 weeks of age or younger) do not mount a delayed-type hypersensitivity (DTH) response due to the failure of a macrophage antigen-presenting cell (APC) to induce TDTH effector cells. SJL mice that are 10 weeks of age or older produce a normal DTH response. This genetic defect provides a model for the investigation of functional subpopulations of APC which interact with specific subpopulations of T cells. In this study, we used this model to examine whether macrophage APC impairment involves APC-dependent immune responses other than DTH. No age-dependent differences were found in the ability of spleen cells from SJL mice to proliferate and synthesize interleukin-2 in response to concanavalin A; nor was the proliferative response to a variety of antigenic stimuli affected. In addition, no differences were observed in the contact sensitivity response or in the in vitro generation of allogeneic cytotoxic T lymphocytes (CTL). In contrast, the in vivo generation of allogeneic CTL was significantly depressed in 6-week-old SJL and could not be restored to normal by the adoptive transfer of macrophages from DTH responsive 12-week-old SJL mice. Finally, examination of the humoral response of 6-week-old SJL indicated no impairment in IgM or IgG serum antibody levels or in the induction of splenic B cells. Thus, the macrophage APC regulating the induction of TDTH effector cells does not appear to participate in the induction of T helper cells for other cellular and humoral responses. These data support the hypothesis that distinct subpopulations of APC may regulate the induction of specific immune effector mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=0024560379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024560379&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(89)90233-5

DO - 10.1016/0008-8749(89)90233-5

M3 - Article

VL - 119

SP - 171

EP - 181

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -