Evidence for an endogenous peptide ligand for the phencyclidine receptor

Remi Quirion, Debora A. DiMaggio, Edward D. French, Patricia C. Contreras, Joseph Shiloach, Candace B. Pert, Hilleary Everist, Agu Pert, Thomas L. O'Donohue

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Porcine brain contained an active factor that competed with [3H]-phencyclidine (PCP) for binding to rat brain membranes. On reverse phase high pressure liquid chromatography, the active material eluted between 38-42% acetonitrile. Gel filtration chromatography of the factor predicted a molecular weight of approximately 3000 daltons. The endogenous substance appeared to be selective for PCP receptors as it did not interact with either benzodiazepine, neurotensin, nor with mu, delta, or kappa opioid receptors. The active material showed a heterogenous distribution in brain, with highest concentrations found in hippocampus and cortex. It is likely to be a small peptide since various proteases eliminated or markedly reduced the potency of the compound in a [3H]-PCP binding assay. The material also possessed PCP-like activity in two bioassays. Like PCP, it induced contralateral rotational behavior after unilateral intranigral injection and depressed spontaneous cell activity after iontophoretic micropressure application in hippocampus and cerebral cortex. Thus, this small peptide is likely to be an endogenous ligand for the PCP receptor.

Original languageEnglish (US)
Pages (from-to)967-973
Number of pages7
JournalPeptides
Volume5
Issue number5
DOIs
StatePublished - Jan 1 1984

Keywords

  • Angel dust
  • Behavior
  • Brain
  • Neuropeptide
  • PCP
  • Schizophrenia

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Evidence for an endogenous peptide ligand for the phencyclidine receptor'. Together they form a unique fingerprint.

  • Cite this

    Quirion, R., DiMaggio, D. A., French, E. D., Contreras, P. C., Shiloach, J., Pert, C. B., Everist, H., Pert, A., & O'Donohue, T. L. (1984). Evidence for an endogenous peptide ligand for the phencyclidine receptor. Peptides, 5(5), 967-973. https://doi.org/10.1016/0196-9781(84)90124-4