Evidence for BLM and Topoisomerase IIIα interaction in genomic stability

Peng Hu, Sergey F. Beresten, Anja Van Brabant, Tian Zhang Ye, Pier Paolo Pandolfi, F. Brad Johnson, Leonard Guarente, Nathan Ellis

Research output: Contribution to journalArticle

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Abstract

The genomic instability of persons with Bloom's syndrome (BS) features particularly an increased number of sister-chromatid exchanges (SCEs). The primary cause of the genomic instability is mutation at BLM, which encodes a DNA helicase of the RecQ family. BLM interacts with Topoisomerase IIIα (Topo IIIα), and both BLM and Topo IIIα localize to the nuclear organelles referred to as the promyelocytic leukemia protein (PML) nuclear bodies. In this study we show, by analysis of cells that express various deletion constructs of green fluorescent protein (GFP)-tagged BLM, that the first 133 amino acids of BLM are necessary and sufficient for interaction between Topo IIIα and BLM. The Topo IIIα-interaction domain of BLM is not required for BLM's localization to the PML nuclear bodies; in contrast, Topo IIIα is recruited to the PML nuclear bodies via its interaction with BLM. Expression of a full-length BLM (amino acids 1-1417) in BS cells can correct their high SCEs to normal levels, whereas expression of a BLM fragment that lacks the Topo IIIα interaction domain (amino acids 133-1417) results in intermediate SCE levels. The deficiency of amino acids 133-1417 in the reduction of SCEs was not explained by a defect in DNA helicase activity, because immunoprecipitated 133-1417 protein had 4-fold higher activity than GFP-BLM. The data implicate the BLM-Topo IIIα complex in the regulation of recombination in somatic cells.

Original languageEnglish (US)
Pages (from-to)1287-1298
Number of pages12
JournalHuman Molecular Genetics
Volume10
Issue number12
StatePublished - Jun 1 2001
Externally publishedYes

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Genomic Instability
Sister Chromatid Exchange
Bloom Syndrome
Amino Acids
Green Fluorescent Proteins
RecQ Helicases
DNA Helicases
DNA topoisomerase III
Organelles
Genetic Recombination
Mutation
Promyelocytic Leukemia Protein
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Hu, P., Beresten, S. F., Van Brabant, A., Ye, T. Z., Pandolfi, P. P., Johnson, F. B., ... Ellis, N. (2001). Evidence for BLM and Topoisomerase IIIα interaction in genomic stability. Human Molecular Genetics, 10(12), 1287-1298.

Evidence for BLM and Topoisomerase IIIα interaction in genomic stability. / Hu, Peng; Beresten, Sergey F.; Van Brabant, Anja; Ye, Tian Zhang; Pandolfi, Pier Paolo; Johnson, F. Brad; Guarente, Leonard; Ellis, Nathan.

In: Human Molecular Genetics, Vol. 10, No. 12, 01.06.2001, p. 1287-1298.

Research output: Contribution to journalArticle

Hu, P, Beresten, SF, Van Brabant, A, Ye, TZ, Pandolfi, PP, Johnson, FB, Guarente, L & Ellis, N 2001, 'Evidence for BLM and Topoisomerase IIIα interaction in genomic stability', Human Molecular Genetics, vol. 10, no. 12, pp. 1287-1298.
Hu P, Beresten SF, Van Brabant A, Ye TZ, Pandolfi PP, Johnson FB et al. Evidence for BLM and Topoisomerase IIIα interaction in genomic stability. Human Molecular Genetics. 2001 Jun 1;10(12):1287-1298.
Hu, Peng ; Beresten, Sergey F. ; Van Brabant, Anja ; Ye, Tian Zhang ; Pandolfi, Pier Paolo ; Johnson, F. Brad ; Guarente, Leonard ; Ellis, Nathan. / Evidence for BLM and Topoisomerase IIIα interaction in genomic stability. In: Human Molecular Genetics. 2001 ; Vol. 10, No. 12. pp. 1287-1298.
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