Evidence for the expression of Ia (H 2 associated) antigens on thymus derived lymphocytes

Jeffrey A Frelinger, J. E. Niederhuber, C. S. David, D. C. Shreffler

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

The authors demonstrated in an anti Ia serum the presence of specific antibodies reacting within T cells, as well as with B cells, using a highly sensitive dye exclusion test. This antiserum reacts with both spleen and lymph node in a characteristic biphasic titration curve killing up to 70% of these cells. It also reacts with cortisone resistant thymocytes. The A.TH α A.TL serum can be absorbed with spleen, lymph node, cortisone resistant thymus, or normal thymus cells. Further in vivo absorptions in BALB/c nude cannot remove all of the cytotoxic activity for normal BALB lymph node lymphocytes, while completely removing the activity for nude cells. A Thy 1 positive cell line derived from a C57Br leukemia is reactive with this anti Ia serum.

Original languageEnglish (US)
Pages (from-to)1273-1284
Number of pages12
JournalJournal of Experimental Medicine
Volume140
Issue number5
StatePublished - 1974
Externally publishedYes

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H-2 Antigens
Thymus Gland
Lymph Nodes
Cortisone
Lymphocytes
Spleen
Serum
Thymocytes
Immune Sera
Leukemia
B-Lymphocytes
Coloring Agents
T-Lymphocytes
Cell Line
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Evidence for the expression of Ia (H 2 associated) antigens on thymus derived lymphocytes. / Frelinger, Jeffrey A; Niederhuber, J. E.; David, C. S.; Shreffler, D. C.

In: Journal of Experimental Medicine, Vol. 140, No. 5, 1974, p. 1273-1284.

Research output: Contribution to journalArticle

Frelinger, Jeffrey A ; Niederhuber, J. E. ; David, C. S. ; Shreffler, D. C. / Evidence for the expression of Ia (H 2 associated) antigens on thymus derived lymphocytes. In: Journal of Experimental Medicine. 1974 ; Vol. 140, No. 5. pp. 1273-1284.
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