Evidence of widespread binding of HLA class I molecules to peptides

Jeffrey A Frelinger, Frances M. Gotch, Hans Zweerink, Emma Wain, Andrew J. McMichael

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have tested the binding of HLA class I proteins to peptides using a solid-phase binding assay. We tested 102 peptides, mostly derived from the HIV gag and HIV pol sequences. Most peptides did not bind to any class I protein tested. The pattern of binding among the three class I proteins tested, HLA-A2, -B27, and -B8, was appropriately 85% concordant. Further, all five of the known HIV-1 gag T cell epitopes detected by human CTL bound at least one class I protein. Binding of class I to the peptides could be detected either by directly iodinated class I proteins, or indirectly using monoclonal antibodies specific for class I. The binding to the plates could be blocked with MA2.1, which binds in the α1 region of A2, but not by W6/32, which binds elsewhere. The data presented here show that binding of class I to peptides is specific, but that many peptides bind to more than a single class I protein.

Original languageEnglish (US)
Pages (from-to)827-834
Number of pages8
JournalJournal of Experimental Medicine
Volume172
Issue number3
StatePublished - Sep 1 1990
Externally publishedYes

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Peptides
Proteins
HIV
HLA-A2 Antigen
HLA-B27 Antigen
T-Lymphocyte Epitopes
Immunoglobulin Isotypes
varespladib methyl
HIV-1
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Frelinger, J. A., Gotch, F. M., Zweerink, H., Wain, E., & McMichael, A. J. (1990). Evidence of widespread binding of HLA class I molecules to peptides. Journal of Experimental Medicine, 172(3), 827-834.

Evidence of widespread binding of HLA class I molecules to peptides. / Frelinger, Jeffrey A; Gotch, Frances M.; Zweerink, Hans; Wain, Emma; McMichael, Andrew J.

In: Journal of Experimental Medicine, Vol. 172, No. 3, 01.09.1990, p. 827-834.

Research output: Contribution to journalArticle

Frelinger, JA, Gotch, FM, Zweerink, H, Wain, E & McMichael, AJ 1990, 'Evidence of widespread binding of HLA class I molecules to peptides', Journal of Experimental Medicine, vol. 172, no. 3, pp. 827-834.
Frelinger JA, Gotch FM, Zweerink H, Wain E, McMichael AJ. Evidence of widespread binding of HLA class I molecules to peptides. Journal of Experimental Medicine. 1990 Sep 1;172(3):827-834.
Frelinger, Jeffrey A ; Gotch, Frances M. ; Zweerink, Hans ; Wain, Emma ; McMichael, Andrew J. / Evidence of widespread binding of HLA class I molecules to peptides. In: Journal of Experimental Medicine. 1990 ; Vol. 172, No. 3. pp. 827-834.
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