Evolution of insect metamorphosis: A microarray-based study of larval and adult gene expression in the ant Camponotus festinatus

Michael A D Goodisman, Jun Isoe, Diana E Wheeler, Michael A. Wells

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Holometabolous insects inhabit almost every terrestrial ecosystem. The evolutionary success of holometabolous insects stems partly from their developmental program, which includes discrete larval and adult stages. To gain an understanding of how development differs among holometabolous insect taxa, we used cDNA microarray technology to examine differences in gene expression between larval and adult Camponotus festinatus ants. We then compared expression patterns obtained from our study to those observed in the fruitfly Drosophila melanogaster. We found that many genes showed distinct patterns of expression between the larval and adult ant life stages, a result that was confirmed through quantitative reverse-transcriptase polymerase chain reaction. Genes involved in protein metabolism and possessing structural activity tended to be more highly expressed in larval than adult ants. In contrast, genes relatively upregulated in adults possessed a greater diversity of functions and activities. We also discovered that patterns of expression observed for homologous genes in D. melanogaster differed substantially from those observed in C. festinatus. Our results suggest that the specific molecular mechanisms involved in metamorphosis will differ substantially between insect taxa. Systematic investigation of gene expression during development of other taxa will provide additional information on how developmental pathways evolve.

Original languageEnglish (US)
Pages (from-to)858-870
Number of pages13
JournalEvolution
Volume59
Issue number4
StatePublished - Apr 2005

Fingerprint

Camponotus
Ants
metamorphosis
gene expression
ant
Insects
Formicidae
insect
Gene Expression
insects
Drosophila melanogaster
gene
Genes
genes
microarray technology
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Ecosystem
fruit flies
protein metabolism

Keywords

  • Camponotus festinatus
  • cDNA microarray
  • Developmental regulation of expression
  • Drosophila melanogaster
  • Eusocial
  • Formicidae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Genetics(clinical)
  • Ecology
  • Genetics

Cite this

Evolution of insect metamorphosis : A microarray-based study of larval and adult gene expression in the ant Camponotus festinatus. / Goodisman, Michael A D; Isoe, Jun; Wheeler, Diana E; Wells, Michael A.

In: Evolution, Vol. 59, No. 4, 04.2005, p. 858-870.

Research output: Contribution to journalArticle

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