Ex vivo fetal whole ovarian culture model: An essential tool for studies in reproductive toxicology and pharmacology

Jone A. Stanley, Joe A. Arosh, Patricia B Hoyer, Sakhila K. Banu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Major limitations in understanding the direct effects of endocrine-disrupting chemicals (EDCs) and cell signaling events in ovarian cellular dynamics in mammals include a lack of proper and simple tools/techniques as well as gaps in knowledge regarding the critical window(s) of vulnerability. Identifying and validating such tools and evaluating the effects of EDCs on molecular dynamics and cellular events during the critical windows of ovarian development are very important to improve the fertility in women and preserve the future health of the developing fetuses. Therefore, we developed a fetal whole ovarian ex vivo culture model. Ex vivo ovary culture models allow varying culture parameters in a highly controlled manner and thus have the potential to allow a more thorough evaluation for reproductive toxicity studies and drug response. This chapter describes clear and thorough details for setting up and maintaining an ex vivo culture system from the rat ovaries and further analyses of mRNA and protein expressions and estimating follicle numbers.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-127
Number of pages21
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1965
ISSN (Print)1064-3745

Keywords

  • Ex vivo culture
  • Fetal ovary
  • Follicle number
  • Immunohistochemistry
  • RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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