Ex vivo optical coherence tomography and laser induced fluorescence spectroscopy imaging of murine gastrointestinal tract

Lida Hariri, Alexandre R. Tumlinson, Norman Wade, David Besselsen, Urs Utzinger, Eugene Gerner, Jennifer Barton

Research output: Contribution to journalConference article

Abstract

Optical Coherence Tomography (OCT) and Laser Induced Fluorescence Spectroscopy (LIF) have separately been found to have clinical potential in identifying human gastrointestinal (GI) pathologies, yet their diagnostic capability in mouse models of human disease is unknown. We combine the two modalities to survey the GI tract of a variety of mouse strains and sample dysplasias and inflammatory bowel disease (IBD) of the small and large intestine. Segments of duodenum and lower colon 2.5 cm in length and the entire esophagus from 10 mice each of two colon cancer models (ApcMin and AOM treated A/J) and two IBD models (Il-2 and Il-10) and 5 mice each of their respective controls were excised. OCT images and LIF spectra were obtained simultaneously from each tissue sample within 1 hour of extraction. Histology was used to classify tissue regions as normal, Peyer's patch, dysplasia, adenoma, or IBD. Features in corresponding regions of OCT images were analyzed. Spectra from each of these categories were averaged and compared via the student's t-test. Features in OCT images correlated to histology in both normal and diseased tissue samples. In the diseased samples, OCT was able to identify early stages of mild colitis and dysplasia. In the sample of IBD, the LIF spectra displayed unique peaks at 635nm and 670nm, which were attributed to increased porphyrin production in the proliferating bacteria of the disease. These peaks have the potential to act as a diagnostic for IBD. OCT and LIF appear to be useful and complementary modalities for imaging mouse models.

Original languageEnglish (US)
Article number52
Pages (from-to)295-306
Number of pages12
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5692
DOIs
StatePublished - Jul 21 2005
EventAdvanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States
Duration: Jan 23 2005Jan 26 2005

Keywords

  • Animal Models
  • Colorectal Disease
  • Gastrointestinal Disease
  • Gastrointestinal Tract
  • LIF
  • Mouse Anatomy
  • OCT

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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