EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster

Linda S Powers, H. Schagger, G. von^Jagow, J. Smith, B. Chance, T. Ohnishi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, Mössbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome {itbc1 complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.

Original languageEnglish (US)
Pages (from-to)293-298
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume975
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Nitrogen
Ligands
Thermus thermophilus
Heart Mitochondria
Mitochondria
Fees and Charges
Electron Spin Resonance Spectroscopy
Cytochromes
Cysteine
Paramagnetic resonance
Assays
Proteins

Keywords

  • (Bovine heart)
  • Cytochrome bc
  • Iron-sulfur cluster
  • Nitrogen ligand
  • Rieske protein

ASJC Scopus subject areas

  • Biophysics

Cite this

EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster. / Powers, Linda S; Schagger, H.; von^Jagow, G.; Smith, J.; Chance, B.; Ohnishi, T.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 975, No. 2, 1989, p. 293-298.

Research output: Contribution to journalArticle

Powers, Linda S ; Schagger, H. ; von^Jagow, G. ; Smith, J. ; Chance, B. ; Ohnishi, T. / EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster. In: Biochimica et Biophysica Acta - Bioenergetics. 1989 ; Vol. 975, No. 2. pp. 293-298.
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AU - Chance, B.

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