Examination of the structure-toxicity relationships of l-cysteine-S-conjugates of halogenated alkenes and their corresponding mercapturic acids in rat renal tissue slices

G. J. Stijntjes, J. N M Commandeur, J. M. te Koppele, S. McGuinness, A Jay Gandolfi, N. P E Vermeulen

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Abstract

Rat kidney slices were produced using a modified version of a mechanical tissue slicer. The slices were incubated with various concentrations of l-cysteine conjugates and mercapturic acids of halogenated alkenes in a submersion incubation system. The slices showed a time- and concentration-dependent toxicity to the nephrotoxic conjugates. The five l-cysteine conjugates tested: S-(1,2-dichlorovinyl)-l-cysteine (1,2-DCVC), S-(1,1,2,2-tetrafluoroethyl)-l-cysteine (TFEC), S-(1-chloro-1,2,2-trifluoroethyl)-l-cysteine (CTFEC), S-(1,1-dichloro-2,2-difluoroethyl)-l-cysteine (DCDFEC) and S-(1,1, dibromo-2,2-difluoroethyl)-l-cysteine (DBDFEC) were more toxic compared to the corresponding mercapturic acids. Comparing the in vitro toxicity data with the in vivo data for the same compounds results in similar ranking for the relative nephrotoxicity of the conjugates.

Original languageEnglish (US)
Pages (from-to)67-79
Number of pages13
JournalToxicology
Volume79
Issue number1
DOIs
StatePublished - Mar 30 1993

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Acetylcysteine
Alkenes
Cysteine
Toxicity
Rats
Tissue
Kidney
Poisons
Immersion

Keywords

  • Halogenated alkenes
  • l-cysteine conjugates
  • Mercapturic acids
  • Nephrotoxicity
  • Rat renal slice

ASJC Scopus subject areas

  • Toxicology

Cite this

Examination of the structure-toxicity relationships of l-cysteine-S-conjugates of halogenated alkenes and their corresponding mercapturic acids in rat renal tissue slices. / Stijntjes, G. J.; Commandeur, J. N M; te Koppele, J. M.; McGuinness, S.; Gandolfi, A Jay; Vermeulen, N. P E.

In: Toxicology, Vol. 79, No. 1, 30.03.1993, p. 67-79.

Research output: Contribution to journalArticle

Stijntjes, G. J. ; Commandeur, J. N M ; te Koppele, J. M. ; McGuinness, S. ; Gandolfi, A Jay ; Vermeulen, N. P E. / Examination of the structure-toxicity relationships of l-cysteine-S-conjugates of halogenated alkenes and their corresponding mercapturic acids in rat renal tissue slices. In: Toxicology. 1993 ; Vol. 79, No. 1. pp. 67-79.
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