Excitatory amino acid profiles of synovial fluid from patients with arthritis

Terry McNearney, Debbie Speegle, Nada Lawand, Jeffrey R Lisse, Karin N. Westlund

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Objective. Previous studies in an experimental synovitis model in rats determined that administration of glutamate and aspartate into the joint produces hyperalgesic responses, while their receptor antagonists provide protection against the development of a hyperalgesic state. We examined concentrations of amino acids in synovial fluid (SF) to determine if increases might be relevant to human joint pathology. Methods. One hundred forty-four repository SF samples from patients undergoing diagnostic or therapeutic arthrocentesis and 14 SF samples from 7 cadavers were analyzed by high pressure liquid chromatography and compared as arthritic and control cohorts. Results. Compared to the average concentrations from the autopsy cases, the excitatory amino acids (EAA) glutamate and aspartate in SF from patients with synovitis were 54 and 28 times higher, respectively. Increases for all other amino acids ranged from 3 to 18-fold. The values for glutamate and aspartate were significantly higher than the mean increase for other amino acids compared using unpaired t tests (p < 0.0001). The mean ratio of glutamate and aspartate elevations over the mean increase for other amino acids was 4-fold and 2-fold, respectively. The EAA were highest in Reiter's, infectious arthropathies, and systemic lupus erythematosus, but did not appreciably segregate to diagnosis or SF white blood cell count. Conclusion. Our data provide evidence of increased glutamate and aspartate in the SF of humans with active arthritis, suggesting that glutamate mediated events may contribute to the pathogenesis of human arthritic conditions.

Original languageEnglish (US)
Pages (from-to)739-745
Number of pages7
JournalJournal of Rheumatology
Volume27
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Excitatory Amino Acids
Synovial Fluid
Arthritis
Glutamic Acid
Aspartic Acid
Amino Acids
Synovitis
Joints
Joint Diseases
Leukocyte Count
Cadaver
Systemic Lupus Erythematosus
Autopsy
Theoretical Models
High Pressure Liquid Chromatography
Pathology

Keywords

  • Arthritis
  • Aspartate
  • Glutamate
  • Hyperalgesia
  • Synovial fluid

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

McNearney, T., Speegle, D., Lawand, N., Lisse, J. R., & Westlund, K. N. (2000). Excitatory amino acid profiles of synovial fluid from patients with arthritis. Journal of Rheumatology, 27(3), 739-745.

Excitatory amino acid profiles of synovial fluid from patients with arthritis. / McNearney, Terry; Speegle, Debbie; Lawand, Nada; Lisse, Jeffrey R; Westlund, Karin N.

In: Journal of Rheumatology, Vol. 27, No. 3, 2000, p. 739-745.

Research output: Contribution to journalArticle

McNearney, T, Speegle, D, Lawand, N, Lisse, JR & Westlund, KN 2000, 'Excitatory amino acid profiles of synovial fluid from patients with arthritis', Journal of Rheumatology, vol. 27, no. 3, pp. 739-745.
McNearney, Terry ; Speegle, Debbie ; Lawand, Nada ; Lisse, Jeffrey R ; Westlund, Karin N. / Excitatory amino acid profiles of synovial fluid from patients with arthritis. In: Journal of Rheumatology. 2000 ; Vol. 27, No. 3. pp. 739-745.
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