Exercise training, glucose transporters, and glucose transport in rat skeletal muscles

K. J. Rodnick, Erik J Henriksen, D. E. James, J. O. Holloszy

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

It was previously found that voluntary wheel running induces an increase in the insulin-sensitive glucose transporter, i.e., the GLUT4 isoform, in rat plantaris muscle (K.J. Rodnick, J.O. Holloszy, C.E. Mondon, and D.E. James. Diabetes 39: 1425-1429, 1990). The present study was undertaken to determine whether 1) the increase in muscle GLUT4 protein is associated with an increase in maximally stimulated glucose transport activity, 2) a conversion of type IIb to type IIa or type I muscle fibers plays a role in the increase in GLUT4 protein, and 3) an increase in the GLUT1 isoform is a component of the adaptation of muscle to endurance exercise. Five weeks of voluntary wheel running that resulted in a 33% increase in citrate synthase activity induced a 50% increase in GLUT4 protein in epitrochlearis muscles of female Sprague-Dawley rats. The rate of 2-deoxyglucose transport maximally stimulated with insulin or insulin plus contractions was increased ~40% (P < 0.05). There was no change in muscle fiber type composition, evaluated by myosin ATPase staining, in the epitrochlearis. There was also no change in GLUT1 protein concentration. We conclude that an increase in GLUT4, but not of GLUT1 protein, is a component of the adaptive response of muscle to endurance exercise and that the increase in GLUT4 protein is associated with an increased capacity for glucose transport.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number1 31-1
StatePublished - 1992
Externally publishedYes

Fingerprint

Glucose Transporter Type 4
Facilitative Glucose Transport Proteins
Muscle
Rats
Skeletal Muscle
Glucose Transporter Type 1
Exercise
Glucose
Muscles
Insulin
Running
Protein Isoforms
Slow-Twitch Muscle Fibers
Citrate (si)-Synthase
Muscle Proteins
Deoxyglucose
Myosins
Wheels
Durability
Sprague Dawley Rats

Keywords

  • Epitrochlearis
  • GLUT4
  • Soleus
  • Voluntary wheel running

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Exercise training, glucose transporters, and glucose transport in rat skeletal muscles. / Rodnick, K. J.; Henriksen, Erik J; James, D. E.; Holloszy, J. O.

In: American Journal of Physiology - Cell Physiology, Vol. 262, No. 1 31-1, 1992.

Research output: Contribution to journalArticle

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