Exogenous γ-aminobutyric acid (GABA) affects pollen tube growth via modulating putative Ca2+-permeable membranechannels and is coupled to negative regulation on glutamate decarboxylase

Guang Hui Yu, Jie Zou, Jing Feng, Xiong Bo Peng, Ju You Wu, Ying Liang Wu, Ravishankar Palanivelu, Meng Xiang Sun

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

γ-Aminobutyric acid (GABA) is implicated in pollen tube growth, but the molecular and cellular mechanisms that it mediates are largely unknown. Here, it is shown that exogenous GABA modulates putative Ca2+- permeable channels on the plasma membranes of tobacco pollen grains and pollen tubes. Whole-cell voltage-clamp experiments and noninvasive micromeasurement technology (NMT) revealed that the influx of Ca2+ increases in pollen tubes in response to exogenous GABA. It is also demonstrated that glutamate decarboxylase (GAD), the rate-limiting enzyme of GABA biosynthesis, is involved in feedback controls of Ca2+-permeable channels to fluctuate intracellular GABA levels and thus modulate pollen tube growth. The findings suggest that GAD activity linked with Ca2+-permeable channels relays an extracellular GABA signal and integrates multiple signal pathways to modulate tobacco pollen tube growth. Thus, the data explain how GABA mediates the communication between the style and the growing pollen tubes.

Original languageEnglish (US)
Pages (from-to)3235-3248
Number of pages14
JournalJournal of Experimental Botany
Volume65
Issue number12
DOIs
StatePublished - Jul 2014

Keywords

  • Cell-cell communication
  • Glutamate decarboxylase
  • Nicotiana tabacum
  • Pollen tube
  • γ-aminobutyric acid

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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