Exon-based mapping of microarray probes: Recovering differential gene expression signal in underpowered hypoxia experiment

Dmitry N. Grigoryev, Shwu Fan Ma, Larissa A. Shimoda, Roger A. Johns, Byungkook Lee, Joe GN Garcia

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

There is an immense collection of underpowered Affymetrix gene array experiments. Although a majority of these experiments generated biologically feasible results, the considerable fraction of assays failed to identify expected transcriptional changes. There is an unused potential of Affymetrix probe-set redundancy for common exonic and UTR regions. We hypothesized that group analysis of multiple probe-sets which hybridize to the same exon or UTR will increase array discriminating power of transcriptional changes. To test this hypothesis, we analyzed Affymetrix mouse probe-sets that share the same exon using blocking feature of the Significance Analysis of Microarrays (SAM). Two-thousand two-hundred one exon-sharing probe-sets targeting 1011 transcripts were identified by mapping 36701 MG-U74v2 probe-sets to genomic alignments of 3,971,086 known mouse transcripts. Using the blocking feature of SAM with an underpowered (two microarrays per experimental condition) mouse hypoxia-induced pulmonary hypertension model, we identified 24 genes that were significantly (FDR<5%) affected by hypoxia but were not detected by regular SAM. The relevance of the four newly identified genes (Mig6, F3, Bmp6, and Ndrg1) to known hypoxia-associated responses was confirmed by PubMatrix; and hypoxia-induced up-regulation of Mig6 expression was validated by real-time RT-PCR. We demonstrated that analysis of exon-sharing probe-sets allowed discovery of additional hypoxia-affected genes in an underpowered array experiment. This method will facilitate re-evaluation of existing underpowered Affymetrix gene expression profiles.

Original languageEnglish (US)
Pages (from-to)134-139
Number of pages6
JournalMolecular and Cellular Probes
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 2007
Externally publishedYes

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Keywords

  • Gene expression profiling
  • Hypoxia
  • Microarray
  • Mouse model
  • Oligonucleotide probe
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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