Abstract
Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple α2-adrenergic receptor (α2 AR) subtypes. We have cloned a human α2AR by using the polymerase chain reaction with oligonucleotide primers homologous to conserved regions of the previously cloned α2ARs, the genes for which are located on human chromosomes 4 (C4) and 10 (C10). The deduced amino acid sequence encodes a protein of 450 amino acids whose putative topology is similar to that of the family of guanine nucleotide-binding protein-coupled receptors, but whose structure most closely resembles that of the α2ARs. Competition curve analysis of the binding properties of the receptor expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique α2AR pharmacology. Hybridization with somatic cell hybrids shows that the gene for this receptor is located on chromosome 2. Northern blot analysis of various rat tissues shows expression in liver and kidney. The unique pharmacology and tissue localization of this receptor suggest that this is an α2AR subtype not previously identified by classical pharmacological or ligand binding approaches.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5094-5098 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 87 |
| Issue number | 13 |
| State | Published - 1990 |
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Keywords
- α-adrenoceptor
- [H]yohimbine binding
- Catecholamines
- G protein
- Polymerase chain reaction
ASJC Scopus subject areas
- Genetics
- General
Cite this
Expansion of the α2-adrenergic receptor family : Cloning and characterization of a human α2-adrenergic receptor subtype, the gene for which is located on chromosome 2. / Lomasney, Jon W.; Lorenz, Wulfing; Allen, Lee F.; Klng, Klim; Regan, John W; Yang-Fengh, Theresa L.; Caron, Marc G.; Lefkowitz, Robert J.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 13, 1990, p. 5094-5098.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expansion of the α2-adrenergic receptor family
T2 - Cloning and characterization of a human α2-adrenergic receptor subtype, the gene for which is located on chromosome 2
AU - Lomasney, Jon W.
AU - Lorenz, Wulfing
AU - Allen, Lee F.
AU - Klng, Klim
AU - Regan, John W
AU - Yang-Fengh, Theresa L.
AU - Caron, Marc G.
AU - Lefkowitz, Robert J.
PY - 1990
Y1 - 1990
N2 - Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple α2-adrenergic receptor (α2 AR) subtypes. We have cloned a human α2AR by using the polymerase chain reaction with oligonucleotide primers homologous to conserved regions of the previously cloned α2ARs, the genes for which are located on human chromosomes 4 (C4) and 10 (C10). The deduced amino acid sequence encodes a protein of 450 amino acids whose putative topology is similar to that of the family of guanine nucleotide-binding protein-coupled receptors, but whose structure most closely resembles that of the α2ARs. Competition curve analysis of the binding properties of the receptor expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique α2AR pharmacology. Hybridization with somatic cell hybrids shows that the gene for this receptor is located on chromosome 2. Northern blot analysis of various rat tissues shows expression in liver and kidney. The unique pharmacology and tissue localization of this receptor suggest that this is an α2AR subtype not previously identified by classical pharmacological or ligand binding approaches.
AB - Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple α2-adrenergic receptor (α2 AR) subtypes. We have cloned a human α2AR by using the polymerase chain reaction with oligonucleotide primers homologous to conserved regions of the previously cloned α2ARs, the genes for which are located on human chromosomes 4 (C4) and 10 (C10). The deduced amino acid sequence encodes a protein of 450 amino acids whose putative topology is similar to that of the family of guanine nucleotide-binding protein-coupled receptors, but whose structure most closely resembles that of the α2ARs. Competition curve analysis of the binding properties of the receptor expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique α2AR pharmacology. Hybridization with somatic cell hybrids shows that the gene for this receptor is located on chromosome 2. Northern blot analysis of various rat tissues shows expression in liver and kidney. The unique pharmacology and tissue localization of this receptor suggest that this is an α2AR subtype not previously identified by classical pharmacological or ligand binding approaches.
KW - α-adrenoceptor
KW - [H]yohimbine binding
KW - Catecholamines
KW - G protein
KW - Polymerase chain reaction
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M3 - Article
C2 - 2164221
AN - SCOPUS:0025279114
VL - 87
SP - 5094
EP - 5098
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 13
ER -