Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device

W. Ma, D. Liu, H. Shagoshtasbi, A. Shukla, E. S. Nugroho, Yitshak Zohar, Y. K. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Microfiltration is an important microfluidic technique suitable for enrichment and isolation of cells. However, cell lysing could occur due to hydrodynamic damage that may be detrimental for medical diagnostics. Therefore, we conducted a systematic study of hydrodynamic cell lysing in a high-throughput Circular Multi-Channel Microfiltration (CMCM) device integrated with a polycarbonate membrane. HeLa cells (cervical cancer cells) were driven into the CMCM at different flow rates. The viability of the cells in the CMCM was examined by fluorescence microscopy using Acridine Orange (AO)/Ethidium Bromide (EB) as a marker for viable/dead cells. A simple analytical cell viability model was derived and a 3D numerical model was constructed to examine the correlation of between cell lysing and applied shear stress under varying flow rate and Reynolds number. The measured cell viability as a function of the shear stress was consistent with theoretical and numerical predictions when accounting for cell size distribution.

Original languageEnglish (US)
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages412-415
Number of pages4
DOIs
StatePublished - 2013
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
Duration: Apr 7 2013Apr 10 2013

Other

Other8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
CountryChina
CitySuzhou
Period4/7/134/10/13

Fingerprint

Microfiltration
Hydrodynamics
Theoretical Models
Cells
Throughput
Equipment and Supplies
polycarbonate
Cell Survival
Shear stress
Neoplasms
Flow rate
Acridine Orange
Ethidium
Fluorescence microscopy
Microfluidics
Cell Separation
Numerical models
Reynolds number
Cell Size
HeLa Cells

Keywords

  • cell lysing
  • cell viability
  • enrichment
  • hydrodynamic shear
  • isolation
  • microfiltration
  • microfluidics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biotechnology

Cite this

Ma, W., Liu, D., Shagoshtasbi, H., Shukla, A., Nugroho, E. S., Zohar, Y., & Lee, Y. K. (2013). Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 (pp. 412-415). [6559761] https://doi.org/10.1109/NEMS.2013.6559761

Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. / Ma, W.; Liu, D.; Shagoshtasbi, H.; Shukla, A.; Nugroho, E. S.; Zohar, Yitshak; Lee, Y. K.

8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. p. 412-415 6559761.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ma, W, Liu, D, Shagoshtasbi, H, Shukla, A, Nugroho, ES, Zohar, Y & Lee, YK 2013, Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. in 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013., 6559761, pp. 412-415, 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013, Suzhou, China, 4/7/13. https://doi.org/10.1109/NEMS.2013.6559761
Ma W, Liu D, Shagoshtasbi H, Shukla A, Nugroho ES, Zohar Y et al. Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. p. 412-415. 6559761 https://doi.org/10.1109/NEMS.2013.6559761
Ma, W. ; Liu, D. ; Shagoshtasbi, H. ; Shukla, A. ; Nugroho, E. S. ; Zohar, Yitshak ; Lee, Y. K. / Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. pp. 412-415
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