Experimental characterization of methanol-acetic acid fixative sessile drop dynamics in dry and humid air by video imaging and interference analysis

Research output: Contribution to journalArticle

Abstract

Dynamics of methanol and acetic acid (3:1, v/v) fixative sessile drop is important for metaphase chromosomal spreads in cytogenetic assays. However, it has not been well characterized by biologists from a physical science point of view. In this work, a simple optical setup was built to record the fixative drop spreading and evaporation process. Drop film thickness, cross-sectional profile and surface thinning speed were constructed from the observed interference patterns to show evolution of the process in both dry and humid air. Surface thinning speed analysis at the drop center suggested different evaporation regimes. The ability of characterizing fluid behavior at a scale comparable to the size of cells by interference fringes is expected to facilitate further understanding of the metaphase spreading process at micro- and nano-scale.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume449
Issue number1
DOIs
StatePublished - May 1 2014

Fingerprint

Fixatives
acetic acid
Acetic acid
Acetic Acid
Methanol
methyl alcohol
interference
Imaging techniques
air
Air
evaporation
physical sciences
Evaporation
film thickness
Film thickness
Assays
acids
fluids
profiles
cells

Keywords

  • Cytogenetic assay
  • Interference analysis
  • Marangoni effect
  • Metaphase spread
  • Methanol-acetic acid sessile drop
  • Spreading and evaporation

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

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title = "Experimental characterization of methanol-acetic acid fixative sessile drop dynamics in dry and humid air by video imaging and interference analysis",
abstract = "Dynamics of methanol and acetic acid (3:1, v/v) fixative sessile drop is important for metaphase chromosomal spreads in cytogenetic assays. However, it has not been well characterized by biologists from a physical science point of view. In this work, a simple optical setup was built to record the fixative drop spreading and evaporation process. Drop film thickness, cross-sectional profile and surface thinning speed were constructed from the observed interference patterns to show evolution of the process in both dry and humid air. Surface thinning speed analysis at the drop center suggested different evaporation regimes. The ability of characterizing fluid behavior at a scale comparable to the size of cells by interference fringes is expected to facilitate further understanding of the metaphase spreading process at micro- and nano-scale.",
keywords = "Cytogenetic assay, Interference analysis, Marangoni effect, Metaphase spread, Methanol-acetic acid sessile drop, Spreading and evaporation",
author = "Jian Gu and Frederic Zenhausern",
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AU - Zenhausern, Frederic

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