Experimental study on cryotherapy for fungal corneal ulcer

Yingxin Chen, Weijia Yang, Minghong Gao, Michael W Belin, Hai Yu, Jing Yu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer. Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes. Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst. Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

Original languageEnglish (US)
Article number29
JournalBMC Ophthalmology
Volume15
Issue number1
DOIs
StatePublished - Dec 12 2015

Fingerprint

Corneal Ulcer
Cryotherapy
Therapeutics
Transmission Electron Microscopy
Electron Scanning Microscopy
Fungal Structures
Rabbits
Periodic Acid
Corneal Transplantation
Keratitis
Mycelium
Vision Disorders
Cellular Structures
Aspergillus
Hematoxylin
Eosine Yellowish-(YS)
Candida albicans
Confocal Microscopy
Cornea
Fungi

Keywords

  • Anti-fungal agents
  • Cryotherapy
  • Fungal corneal keratitis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Experimental study on cryotherapy for fungal corneal ulcer. / Chen, Yingxin; Yang, Weijia; Gao, Minghong; Belin, Michael W; Yu, Hai; Yu, Jing.

In: BMC Ophthalmology, Vol. 15, No. 1, 29, 12.12.2015.

Research output: Contribution to journalArticle

Chen, Yingxin ; Yang, Weijia ; Gao, Minghong ; Belin, Michael W ; Yu, Hai ; Yu, Jing. / Experimental study on cryotherapy for fungal corneal ulcer. In: BMC Ophthalmology. 2015 ; Vol. 15, No. 1.
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