Exploratory study of ovarian intraepithelial neoplasia

Molly A. Brewer, James Ranger-Moore, Amy Baruche, David S Alberts, Mark Greene, Deborah Thompson, Yun Liu, John Davis, Peter H. Bartels

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: This was an exploratory study to test two hypotheses related to potential epithelial precursors to ovarian cancer: (a) histologically normal ovarian surface epithelium exhibited changes in the nuclear chromatin pattern, which indicate an ovarian abnormality, and (b) such changes were detectable in the ovarian surface epithelium of cancer-free subjects who were at high risk for ovarian cancer. Experimental Design: Ovaries were carefully collected to avoid damage to the surface epithelium. Five-micron-thick histologic sections were cut and stained with H&E. High-resolution images were recorded from the ovarian surface epithelium and from the underlying stroma of ovaries from normal women (10 cases), women at high risk of developing ovarian cancer (7 cases), and histologically normal areas adjacent to ovarian cancer (3 cases). Karyometric features and measurements of nuclear abnormality were computed for 3,390 epithelial nuclei. Discriminant function analyses and unsupervised learning algorithms were employed to define deviations from normal and to identify the subpopulations of nuclei exhibiting these changes. Results: Epithelium from ovaries harboring a malignant lesion had changes in the nuclear chromatin pattern consistent with a second phenotype, which were not visually detected with histopathologic surveillance. This phenotype was also present in the ovaries obtained from women at increased risk of ovarian cancer, suggesting that it may represent a premalignant abnormality. These changes were statistically significant. Conclusion: The observed changes in karyometric features were sufficiently distinct to warrant further study as both diagnostic and prognostic biomarkers for early detection and prevention of ovarian cancer.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Volume14
Issue number2
DOIs
StatePublished - Feb 2005

Fingerprint

Ovarian Neoplasms
Epithelium
Ovary
Neoplasms
Chromatin
Phenotype
Discriminant Analysis
Research Design
Biomarkers
Learning

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Brewer, M. A., Ranger-Moore, J., Baruche, A., Alberts, D. S., Greene, M., Thompson, D., ... Bartels, P. H. (2005). Exploratory study of ovarian intraepithelial neoplasia. Cancer Epidemiology Biomarkers and Prevention, 14(2), 299-305. https://doi.org/10.1158/1055-9965.EPI-04-0212

Exploratory study of ovarian intraepithelial neoplasia. / Brewer, Molly A.; Ranger-Moore, James; Baruche, Amy; Alberts, David S; Greene, Mark; Thompson, Deborah; Liu, Yun; Davis, John; Bartels, Peter H.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 14, No. 2, 02.2005, p. 299-305.

Research output: Contribution to journalArticle

Brewer, MA, Ranger-Moore, J, Baruche, A, Alberts, DS, Greene, M, Thompson, D, Liu, Y, Davis, J & Bartels, PH 2005, 'Exploratory study of ovarian intraepithelial neoplasia', Cancer Epidemiology Biomarkers and Prevention, vol. 14, no. 2, pp. 299-305. https://doi.org/10.1158/1055-9965.EPI-04-0212
Brewer, Molly A. ; Ranger-Moore, James ; Baruche, Amy ; Alberts, David S ; Greene, Mark ; Thompson, Deborah ; Liu, Yun ; Davis, John ; Bartels, Peter H. / Exploratory study of ovarian intraepithelial neoplasia. In: Cancer Epidemiology Biomarkers and Prevention. 2005 ; Vol. 14, No. 2. pp. 299-305.
@article{95fd92f17265437295994b878df10dce,
title = "Exploratory study of ovarian intraepithelial neoplasia",
abstract = "Purpose: This was an exploratory study to test two hypotheses related to potential epithelial precursors to ovarian cancer: (a) histologically normal ovarian surface epithelium exhibited changes in the nuclear chromatin pattern, which indicate an ovarian abnormality, and (b) such changes were detectable in the ovarian surface epithelium of cancer-free subjects who were at high risk for ovarian cancer. Experimental Design: Ovaries were carefully collected to avoid damage to the surface epithelium. Five-micron-thick histologic sections were cut and stained with H&E. High-resolution images were recorded from the ovarian surface epithelium and from the underlying stroma of ovaries from normal women (10 cases), women at high risk of developing ovarian cancer (7 cases), and histologically normal areas adjacent to ovarian cancer (3 cases). Karyometric features and measurements of nuclear abnormality were computed for 3,390 epithelial nuclei. Discriminant function analyses and unsupervised learning algorithms were employed to define deviations from normal and to identify the subpopulations of nuclei exhibiting these changes. Results: Epithelium from ovaries harboring a malignant lesion had changes in the nuclear chromatin pattern consistent with a second phenotype, which were not visually detected with histopathologic surveillance. This phenotype was also present in the ovaries obtained from women at increased risk of ovarian cancer, suggesting that it may represent a premalignant abnormality. These changes were statistically significant. Conclusion: The observed changes in karyometric features were sufficiently distinct to warrant further study as both diagnostic and prognostic biomarkers for early detection and prevention of ovarian cancer.",
author = "Brewer, {Molly A.} and James Ranger-Moore and Amy Baruche and Alberts, {David S} and Mark Greene and Deborah Thompson and Yun Liu and John Davis and Bartels, {Peter H.}",
year = "2005",
month = "2",
doi = "10.1158/1055-9965.EPI-04-0212",
language = "English (US)",
volume = "14",
pages = "299--305",
journal = "Cancer Epidemiology Biomarkers and Prevention",
issn = "1055-9965",
publisher = "American Association for Cancer Research Inc.",
number = "2",

}

TY - JOUR

T1 - Exploratory study of ovarian intraepithelial neoplasia

AU - Brewer, Molly A.

AU - Ranger-Moore, James

AU - Baruche, Amy

AU - Alberts, David S

AU - Greene, Mark

AU - Thompson, Deborah

AU - Liu, Yun

AU - Davis, John

AU - Bartels, Peter H.

PY - 2005/2

Y1 - 2005/2

N2 - Purpose: This was an exploratory study to test two hypotheses related to potential epithelial precursors to ovarian cancer: (a) histologically normal ovarian surface epithelium exhibited changes in the nuclear chromatin pattern, which indicate an ovarian abnormality, and (b) such changes were detectable in the ovarian surface epithelium of cancer-free subjects who were at high risk for ovarian cancer. Experimental Design: Ovaries were carefully collected to avoid damage to the surface epithelium. Five-micron-thick histologic sections were cut and stained with H&E. High-resolution images were recorded from the ovarian surface epithelium and from the underlying stroma of ovaries from normal women (10 cases), women at high risk of developing ovarian cancer (7 cases), and histologically normal areas adjacent to ovarian cancer (3 cases). Karyometric features and measurements of nuclear abnormality were computed for 3,390 epithelial nuclei. Discriminant function analyses and unsupervised learning algorithms were employed to define deviations from normal and to identify the subpopulations of nuclei exhibiting these changes. Results: Epithelium from ovaries harboring a malignant lesion had changes in the nuclear chromatin pattern consistent with a second phenotype, which were not visually detected with histopathologic surveillance. This phenotype was also present in the ovaries obtained from women at increased risk of ovarian cancer, suggesting that it may represent a premalignant abnormality. These changes were statistically significant. Conclusion: The observed changes in karyometric features were sufficiently distinct to warrant further study as both diagnostic and prognostic biomarkers for early detection and prevention of ovarian cancer.

AB - Purpose: This was an exploratory study to test two hypotheses related to potential epithelial precursors to ovarian cancer: (a) histologically normal ovarian surface epithelium exhibited changes in the nuclear chromatin pattern, which indicate an ovarian abnormality, and (b) such changes were detectable in the ovarian surface epithelium of cancer-free subjects who were at high risk for ovarian cancer. Experimental Design: Ovaries were carefully collected to avoid damage to the surface epithelium. Five-micron-thick histologic sections were cut and stained with H&E. High-resolution images were recorded from the ovarian surface epithelium and from the underlying stroma of ovaries from normal women (10 cases), women at high risk of developing ovarian cancer (7 cases), and histologically normal areas adjacent to ovarian cancer (3 cases). Karyometric features and measurements of nuclear abnormality were computed for 3,390 epithelial nuclei. Discriminant function analyses and unsupervised learning algorithms were employed to define deviations from normal and to identify the subpopulations of nuclei exhibiting these changes. Results: Epithelium from ovaries harboring a malignant lesion had changes in the nuclear chromatin pattern consistent with a second phenotype, which were not visually detected with histopathologic surveillance. This phenotype was also present in the ovaries obtained from women at increased risk of ovarian cancer, suggesting that it may represent a premalignant abnormality. These changes were statistically significant. Conclusion: The observed changes in karyometric features were sufficiently distinct to warrant further study as both diagnostic and prognostic biomarkers for early detection and prevention of ovarian cancer.

UR - http://www.scopus.com/inward/record.url?scp=13944276354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13944276354&partnerID=8YFLogxK

U2 - 10.1158/1055-9965.EPI-04-0212

DO - 10.1158/1055-9965.EPI-04-0212

M3 - Article

VL - 14

SP - 299

EP - 305

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

IS - 2

ER -