Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants

Debra A. Stern, Josef Riedler, Dennis Nowak, Charlotte Braun-Fahrlander, Ines Swoboda, Nadja Balic, Kuan Wei Chen, Susanne Vrtala, Hans Grönlund, Marianne van Hage, Rudolf Valenta, Susanne Spitzauer, Erika Von Mutius, Donata Vercelli

Research output: Contribution to journalArticle

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Abstract

Background: IgE synthesis by human B cells results from allergen-dependent, TH2-mediated isotype switching. Exposure to a farming environment protects against IgE responses. Objective: We reconstructed allergen-dependent switching patterns in vivo to identify the level or levels at which farm exposure acts to protect against atopy. Methods: Serum IgG1 to IgG4 and IgE to grass (rPhl p 1 and rPhl p 5), cat (rFel d 1), and mite (rDer p 2) were assessed by means of ELISA in the Allergy and Endotoxin study population (812 children). Farm exposure was defined as currently living on a farm, exposure to stables/farm milk in the first year of life, or both. Results: Farm exposure did not affect allergen-specific IgG2 and IgG3 levels but had complex allergen-specific effects on IgG1, IgG4, and IgE levels. Exposure protected against grass-specific responses at every step along the IgG1/IgG4/IgE switching pathway but had no significant effect on mite responses. Protection from cat responses was concentrated at the IgG1 level. For all allergens, failure to express IgG1 was associated with low prevalence of IgG4 or IgE responses. Notably, coexpression of IgG1, IgG4, and IgE to grass was associated with increased risk of allergic disease and higher IgE levels compared with production of IgG1 and IgE without IgG4, suggesting IgG4 coexpression marks stronger activation of TH2-dependent events. Conclusion: The protective effects of farm exposure were confined to TH2-dependent IgG1, IgG4, and IgE expression and were allergen and switch stage specific, suggesting that distinct mechanisms regulate individual steps within allergen-induced class switching in vivo. Clinical implications: Environmental interventions to prevent IgE expression might need to be tailored to specific allergens.

Original languageEnglish (US)
Pages (from-to)351-358
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume119
Issue number2
DOIs
StatePublished - Feb 2007

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Immunoglobulin Class Switching
Agriculture
Allergens
Immunoglobulin G
Immunoglobulin E
Poaceae
Mites

Keywords

  • allergy
  • Asthma
  • atopy
  • farm exposure
  • IgE
  • IgG
  • immediate hypersensitivity
  • immunoglobulin switch region

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants. / Stern, Debra A.; Riedler, Josef; Nowak, Dennis; Braun-Fahrlander, Charlotte; Swoboda, Ines; Balic, Nadja; Chen, Kuan Wei; Vrtala, Susanne; Grönlund, Hans; van Hage, Marianne; Valenta, Rudolf; Spitzauer, Susanne; Von Mutius, Erika; Vercelli, Donata.

In: Journal of Allergy and Clinical Immunology, Vol. 119, No. 2, 02.2007, p. 351-358.

Research output: Contribution to journalArticle

Stern, DA, Riedler, J, Nowak, D, Braun-Fahrlander, C, Swoboda, I, Balic, N, Chen, KW, Vrtala, S, Grönlund, H, van Hage, M, Valenta, R, Spitzauer, S, Von Mutius, E & Vercelli, D 2007, 'Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants', Journal of Allergy and Clinical Immunology, vol. 119, no. 2, pp. 351-358. https://doi.org/10.1016/j.jaci.2006.10.013
Stern, Debra A. ; Riedler, Josef ; Nowak, Dennis ; Braun-Fahrlander, Charlotte ; Swoboda, Ines ; Balic, Nadja ; Chen, Kuan Wei ; Vrtala, Susanne ; Grönlund, Hans ; van Hage, Marianne ; Valenta, Rudolf ; Spitzauer, Susanne ; Von Mutius, Erika ; Vercelli, Donata. / Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants. In: Journal of Allergy and Clinical Immunology. 2007 ; Vol. 119, No. 2. pp. 351-358.
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abstract = "Background: IgE synthesis by human B cells results from allergen-dependent, TH2-mediated isotype switching. Exposure to a farming environment protects against IgE responses. Objective: We reconstructed allergen-dependent switching patterns in vivo to identify the level or levels at which farm exposure acts to protect against atopy. Methods: Serum IgG1 to IgG4 and IgE to grass (rPhl p 1 and rPhl p 5), cat (rFel d 1), and mite (rDer p 2) were assessed by means of ELISA in the Allergy and Endotoxin study population (812 children). Farm exposure was defined as currently living on a farm, exposure to stables/farm milk in the first year of life, or both. Results: Farm exposure did not affect allergen-specific IgG2 and IgG3 levels but had complex allergen-specific effects on IgG1, IgG4, and IgE levels. Exposure protected against grass-specific responses at every step along the IgG1/IgG4/IgE switching pathway but had no significant effect on mite responses. Protection from cat responses was concentrated at the IgG1 level. For all allergens, failure to express IgG1 was associated with low prevalence of IgG4 or IgE responses. Notably, coexpression of IgG1, IgG4, and IgE to grass was associated with increased risk of allergic disease and higher IgE levels compared with production of IgG1 and IgE without IgG4, suggesting IgG4 coexpression marks stronger activation of TH2-dependent events. Conclusion: The protective effects of farm exposure were confined to TH2-dependent IgG1, IgG4, and IgE expression and were allergen and switch stage specific, suggesting that distinct mechanisms regulate individual steps within allergen-induced class switching in vivo. Clinical implications: Environmental interventions to prevent IgE expression might need to be tailored to specific allergens.",
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AU - Swoboda, Ines

AU - Balic, Nadja

AU - Chen, Kuan Wei

AU - Vrtala, Susanne

AU - Grönlund, Hans

AU - van Hage, Marianne

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