Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase

Zain I Khalpey, K. Kalsi, A. Yuen, J. Karbowska, Z. Kochan, E. M. Slominska, M. Forni, M. Bacci, M. Macherini, P. Batten, M. Lavitrano, M. H. Yacoub, R. T. Smolenski

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Ecto-5′-nucleotidase (E5′ N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of ES′ N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5′ N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5′ N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of ESN decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume24
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

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Keywords

  • Complement
  • Ecto-5′-Nucleotidease
  • Endothelial cells
  • Nucleotides
  • Xenotransplantation

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Khalpey, Z. I., Kalsi, K., Yuen, A., Karbowska, J., Kochan, Z., Slominska, E. M., ... Smolenski, R. T. (2005). Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase. Nucleosides, Nucleotides and Nucleic Acids, 24(4), 271-274. https://doi.org/10.1081/NCN-59707