Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny

Karen B. Onel, Carolyn L. Tucek-Szabo, Dalit Ashany, Elizabeth Lacy, Janko Nikolich-Zugich, Keith B. Elkon

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Mice defective in Fas-mediated apoptosis (lpr phenotype) have an intrinsic B cell abnormality that predisposes them to autoantibody production. To investigate potential roles for the Fas receptor (FasR) in B cell tolerance, FasR expression and function were evaluated at different stages of B cell development. FasR expression was very low or absent on pro- and pre-B cells, but was detected in early B cell lines and was up-regulated following IFN-γ-induced maturation of the pre-B cell line 70-Z. Whereas FasR expression was very low in resting mature sIgM+ B cells, expression was markedly increased following mitogen activation and was also elevated in two mature sIgG+ lymphoma lines. FasR expression correlated strongly with the ability of B cells to undergo Fas-mediated apoptosis. In addition, although Fas did not appear to play a direct role in apoptosis mediated by cross-linking of sIg with anti-IgM, anti-FasR and sublethal concentrations of anti-Ig were additive in the induction of apoptosis in the early B cell line WEHI 231. These findings suggest that the Fas pathway is not involved in the elimination of pro- and pre-B cells, but are compatible with an ancillary role for FasR in the elimination of early B cells and elimination of mature B cells following activation.

Original languageEnglish (US)
Pages (from-to)2940-2947
Number of pages8
JournalEuropean Journal of Immunology
Volume25
Issue number10
StatePublished - Oct 1995
Externally publishedYes

Fingerprint

CD95 Antigens
B-Lymphocytes
B-Lymphoid Precursor Cells
Apoptosis
Cell Line
Mitogens
Autoantibodies
Lymphoma
Phenotype

Keywords

  • APO-1
  • B lymphocytes
  • FasR
  • Systemic lupus erythematosus
  • Tolerance

ASJC Scopus subject areas

  • Immunology

Cite this

Onel, K. B., Tucek-Szabo, C. L., Ashany, D., Lacy, E., Nikolich-Zugich, J., & Elkon, K. B. (1995). Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny. European Journal of Immunology, 25(10), 2940-2947.

Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny. / Onel, Karen B.; Tucek-Szabo, Carolyn L.; Ashany, Dalit; Lacy, Elizabeth; Nikolich-Zugich, Janko; Elkon, Keith B.

In: European Journal of Immunology, Vol. 25, No. 10, 10.1995, p. 2940-2947.

Research output: Contribution to journalArticle

Onel, KB, Tucek-Szabo, CL, Ashany, D, Lacy, E, Nikolich-Zugich, J & Elkon, KB 1995, 'Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny', European Journal of Immunology, vol. 25, no. 10, pp. 2940-2947.
Onel, Karen B. ; Tucek-Szabo, Carolyn L. ; Ashany, Dalit ; Lacy, Elizabeth ; Nikolich-Zugich, Janko ; Elkon, Keith B. / Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny. In: European Journal of Immunology. 1995 ; Vol. 25, No. 10. pp. 2940-2947.
@article{d1ace8d994fc497a9c952149af73c433,
title = "Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny",
abstract = "Mice defective in Fas-mediated apoptosis (lpr phenotype) have an intrinsic B cell abnormality that predisposes them to autoantibody production. To investigate potential roles for the Fas receptor (FasR) in B cell tolerance, FasR expression and function were evaluated at different stages of B cell development. FasR expression was very low or absent on pro- and pre-B cells, but was detected in early B cell lines and was up-regulated following IFN-γ-induced maturation of the pre-B cell line 70-Z. Whereas FasR expression was very low in resting mature sIgM+ B cells, expression was markedly increased following mitogen activation and was also elevated in two mature sIgG+ lymphoma lines. FasR expression correlated strongly with the ability of B cells to undergo Fas-mediated apoptosis. In addition, although Fas did not appear to play a direct role in apoptosis mediated by cross-linking of sIg with anti-IgM, anti-FasR and sublethal concentrations of anti-Ig were additive in the induction of apoptosis in the early B cell line WEHI 231. These findings suggest that the Fas pathway is not involved in the elimination of pro- and pre-B cells, but are compatible with an ancillary role for FasR in the elimination of early B cells and elimination of mature B cells following activation.",
keywords = "APO-1, B lymphocytes, FasR, Systemic lupus erythematosus, Tolerance",
author = "Onel, {Karen B.} and Tucek-Szabo, {Carolyn L.} and Dalit Ashany and Elizabeth Lacy and Janko Nikolich-Zugich and Elkon, {Keith B.}",
year = "1995",
month = "10",
language = "English (US)",
volume = "25",
pages = "2940--2947",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - Expression and function of the murine CD95/ FasR/APO-1 receptor in relation to B cell ontogeny

AU - Onel, Karen B.

AU - Tucek-Szabo, Carolyn L.

AU - Ashany, Dalit

AU - Lacy, Elizabeth

AU - Nikolich-Zugich, Janko

AU - Elkon, Keith B.

PY - 1995/10

Y1 - 1995/10

N2 - Mice defective in Fas-mediated apoptosis (lpr phenotype) have an intrinsic B cell abnormality that predisposes them to autoantibody production. To investigate potential roles for the Fas receptor (FasR) in B cell tolerance, FasR expression and function were evaluated at different stages of B cell development. FasR expression was very low or absent on pro- and pre-B cells, but was detected in early B cell lines and was up-regulated following IFN-γ-induced maturation of the pre-B cell line 70-Z. Whereas FasR expression was very low in resting mature sIgM+ B cells, expression was markedly increased following mitogen activation and was also elevated in two mature sIgG+ lymphoma lines. FasR expression correlated strongly with the ability of B cells to undergo Fas-mediated apoptosis. In addition, although Fas did not appear to play a direct role in apoptosis mediated by cross-linking of sIg with anti-IgM, anti-FasR and sublethal concentrations of anti-Ig were additive in the induction of apoptosis in the early B cell line WEHI 231. These findings suggest that the Fas pathway is not involved in the elimination of pro- and pre-B cells, but are compatible with an ancillary role for FasR in the elimination of early B cells and elimination of mature B cells following activation.

AB - Mice defective in Fas-mediated apoptosis (lpr phenotype) have an intrinsic B cell abnormality that predisposes them to autoantibody production. To investigate potential roles for the Fas receptor (FasR) in B cell tolerance, FasR expression and function were evaluated at different stages of B cell development. FasR expression was very low or absent on pro- and pre-B cells, but was detected in early B cell lines and was up-regulated following IFN-γ-induced maturation of the pre-B cell line 70-Z. Whereas FasR expression was very low in resting mature sIgM+ B cells, expression was markedly increased following mitogen activation and was also elevated in two mature sIgG+ lymphoma lines. FasR expression correlated strongly with the ability of B cells to undergo Fas-mediated apoptosis. In addition, although Fas did not appear to play a direct role in apoptosis mediated by cross-linking of sIg with anti-IgM, anti-FasR and sublethal concentrations of anti-Ig were additive in the induction of apoptosis in the early B cell line WEHI 231. These findings suggest that the Fas pathway is not involved in the elimination of pro- and pre-B cells, but are compatible with an ancillary role for FasR in the elimination of early B cells and elimination of mature B cells following activation.

KW - APO-1

KW - B lymphocytes

KW - FasR

KW - Systemic lupus erythematosus

KW - Tolerance

UR - http://www.scopus.com/inward/record.url?scp=0028799882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028799882&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 2940

EP - 2947

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -