Expression and regulation of mouse Serdin1, a highly conserved cardiac-specific leucine-rich repeat protein

Igor I. Adameyko, Ryan E. Mudry, Nga Rewa M Houston-Cummings, Alexander P. Veselov, Carol Gregorio, Sergei G. Tevosian

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Despite recent progress, the precise mechanisms responsible for vertebrate cardiac development are still enigmatic. Better understanding of cardiac biology and disease necessitates identification and analysis of a full spectrum of regulatory and structural proteins specific to the developing heart. By performing an in silico screen, we identified a cardiac-specific gene we named Serdin1. The Serdin1 gene is conserved, and the message is restricted to the heart in several vertebrate species, thus implicating Serdin1 as an important gene in cardiac development. In situ hybridization confirmed that the Serdin1 message is cardiac-specific in mice as early as embryonic day 8.5. Antibody staining demonstrated predominantly nuclear staining in immortalized cardiac cell lines (P19 and HL-1) and proliferating cultured cardiomyocytes, whereas in vivo SERDIN1 localizes to I bands of the sarcomere. Seven kilobases of the upstream regulatory sequence of Serdin1 is sufficient for cardiac-specific expression. Computer analysis revealed an 80-bp homologous region between the mouse and the human Serdin genes that contains GATA, SRF, and MEF sites. Cardiac specificity and localization patterns suggest that SERDIN1 is intimately integrated with the molecular pathways controlling cardiogenesis in vertebrates.

Original languageEnglish (US)
Pages (from-to)540-552
Number of pages13
JournalDevelopmental Dynamics
Volume233
Issue number2
DOIs
StatePublished - Jun 2005

Fingerprint

Vertebrates
Genes
Staining and Labeling
Sarcomeres
Cardiac Myocytes
Computer Simulation
In Situ Hybridization
Heart Diseases
Cell Line
leucine-rich repeat proteins
Antibodies
Proteins

Keywords

  • Cardiac
  • Cardiomyocytes
  • Embryonic
  • Heart
  • HL-1
  • Leucine rich repeat
  • Mouse
  • Nuclear
  • P19
  • Sarcomere

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Adameyko, I. I., Mudry, R. E., Houston-Cummings, N. R. M., Veselov, A. P., Gregorio, C., & Tevosian, S. G. (2005). Expression and regulation of mouse Serdin1, a highly conserved cardiac-specific leucine-rich repeat protein. Developmental Dynamics, 233(2), 540-552. https://doi.org/10.1002/dvdy.20368

Expression and regulation of mouse Serdin1, a highly conserved cardiac-specific leucine-rich repeat protein. / Adameyko, Igor I.; Mudry, Ryan E.; Houston-Cummings, Nga Rewa M; Veselov, Alexander P.; Gregorio, Carol; Tevosian, Sergei G.

In: Developmental Dynamics, Vol. 233, No. 2, 06.2005, p. 540-552.

Research output: Contribution to journalArticle

Adameyko, Igor I. ; Mudry, Ryan E. ; Houston-Cummings, Nga Rewa M ; Veselov, Alexander P. ; Gregorio, Carol ; Tevosian, Sergei G. / Expression and regulation of mouse Serdin1, a highly conserved cardiac-specific leucine-rich repeat protein. In: Developmental Dynamics. 2005 ; Vol. 233, No. 2. pp. 540-552.
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