Expression of β-actin during progression of mouse skin tumors

Lawrence E. Ostrowski, Peter Krieg, Joanne Finch, Anne E Cress, Raymond B Nagle, G. Tim Bowden

Research output: Contribution to journalArticle

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Abstract

Recombinant DNA techniques have been employed to isolate sequences that are over-expressed during multi-stage carcinogenesis in the mouse skin. One of these six cDNA sequences, mal-4, detected 1.9-KB transcripts that were expressed at levels 10-fold higher in squamous cell carcinomas (SCCs) in comparison to normal epidermis. A full-length cDNA for mal-4 was obtained from a λgt10 cDNA library made from an SCC-producing cell line, PDVC57. DNA sequencing of 1300 out of 1900 bp of the mal-4 cDNA and searching of Genbank showed >95% DNA sequence similarity to mouse β-actin over the entire cDNA and >98% similarity over the 3' untranslated region, which is unique to the various isoforms of actins. Southern analysis showed no amplification or rearrangement of the β-actin gene had occurred during tumor progression. The RNA:RNA hybrid protection assay was utilized to screen for the expression of mutated β-actin(s) in mouse skin tumors. No evidence for mutation was obtained in any of the tumors examined. Fluorescence microscopy of tumor sections stained with rhodamine-conjugated phalloidin showed a peripheral pattern of F-actin localization with no gross differences between papillomas and carcinomas. Approximately equal amounts of β-actin were observed following two-dimensional gel electrophoresis of proteins extracted from normal epidermis, papillomas or SCCs, indicating that the over-expression of β-actin RNA in SCCs did not result in an increased steadystate level of β-actin protein. These results suggest that alterations in actin metabolism accompany tumor progression.

Original languageEnglish (US)
Pages (from-to)1439-1444
Number of pages6
JournalCarcinogenesis
Volume10
Issue number8
DOIs
StatePublished - Aug 1989

Fingerprint

Actin
Progression
Skin
Actins
Mouse
Tumors
Tumor
RNA
CDNA
Neoplasms
Squamous Cell Carcinoma
Complementary DNA
Cell
Epidermis
Proteins
Papilloma
Fluorescence microscopy
DNA sequences
Electrophoresis
Metabolism

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Expression of β-actin during progression of mouse skin tumors. / Ostrowski, Lawrence E.; Krieg, Peter; Finch, Joanne; Cress, Anne E; Nagle, Raymond B; Bowden, G. Tim.

In: Carcinogenesis, Vol. 10, No. 8, 08.1989, p. 1439-1444.

Research output: Contribution to journalArticle

Ostrowski, Lawrence E. ; Krieg, Peter ; Finch, Joanne ; Cress, Anne E ; Nagle, Raymond B ; Bowden, G. Tim. / Expression of β-actin during progression of mouse skin tumors. In: Carcinogenesis. 1989 ; Vol. 10, No. 8. pp. 1439-1444.
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