Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin

Petra Milde, Ulrike Hauser, Thomas Simon, Gerhard Mall, Volker Ernst, Mark R Haussler, Peter Frosch, Ernst W. Rauterberg

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Increasing evidence suggests an immunoregulatory function of the potent steroid hormone 1,25-dihydroxyvitamin D3(1,25(OH)2D3) which has been successfully applied for treatment of psoriasis. The skin is both a site of production and a target of 1,25(OH)2D3. In vitro, 1,25(OH)2D3 inhibits proliferation and stimulates differentiation of keratinocytes. We investigated the in situ expression of vitamin D-receptors (VDR) in normal and psoriatic skin by immunochemical methods. The VDR were visualized using the monoclonal antibody (MoAb) 9A7g to the VDR and the labeled avidinbiotin technique. Immunoreactivity was consistently confined to nuclei in all skin biopsies. In normal skin specimens (n = 10) VDR antigens were expressed in keratinocytes of all epidermal layers (except those of the stratum corneum) and in cells of the epidermal appendages. Double labeling experiments with MoAb to cluster-defined antigens indicated that melanocytes and approximately 75% of Langerhans cells exhibit 1,25(OH)2D3 receptors in normal skin biopsies (n = 5). Depending on their localization in skin compartments 42-62% of CD11b+ positive macrophages and 45-75% of CD3+ T lymphocytes expressed VDR. Non-lesional psoriatic skin specimens (n = 8) revealed nearly identical staining patterns. Lesional psoriatic skin specimens (n = 8) exhibited a significant increase of VDR expression both in basal and suprabasal epidermal layers as measured by computer-assisted morphometry and showed a remarkable change of the immune cell pattern: the densitity and proportion of VDR positive T lymphocytes and macrophages were higher in the epidermal and the perivascular papillary loop compartment. These in vivo findings strongly support the hypothesis that 1,25(OH)2D3 modulates immune response and cell proliferation/differentiation in human skin.

Original languageEnglish (US)
Pages (from-to)230-239
Number of pages10
JournalJournal of Investigative Dermatology
Volume97
Issue number2
StatePublished - Aug 1991

Fingerprint

Calcitriol Receptors
Skin
T-cells
Biopsy
Macrophages
Keratinocytes
Steroid hormones
Monoclonal Antibodies
T-Lymphocytes
Antigens
Langerhans Cells
Calcitriol
Melanocytes
Cell proliferation
Psoriasis
Cornea
Labeling
Cell Differentiation
Steroids
Cell Proliferation

ASJC Scopus subject areas

  • Dermatology

Cite this

Milde, P., Hauser, U., Simon, T., Mall, G., Ernst, V., Haussler, M. R., ... Rauterberg, E. W. (1991). Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin. Journal of Investigative Dermatology, 97(2), 230-239.

Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin. / Milde, Petra; Hauser, Ulrike; Simon, Thomas; Mall, Gerhard; Ernst, Volker; Haussler, Mark R; Frosch, Peter; Rauterberg, Ernst W.

In: Journal of Investigative Dermatology, Vol. 97, No. 2, 08.1991, p. 230-239.

Research output: Contribution to journalArticle

Milde, P, Hauser, U, Simon, T, Mall, G, Ernst, V, Haussler, MR, Frosch, P & Rauterberg, EW 1991, 'Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin', Journal of Investigative Dermatology, vol. 97, no. 2, pp. 230-239.
Milde, Petra ; Hauser, Ulrike ; Simon, Thomas ; Mall, Gerhard ; Ernst, Volker ; Haussler, Mark R ; Frosch, Peter ; Rauterberg, Ernst W. / Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin. In: Journal of Investigative Dermatology. 1991 ; Vol. 97, No. 2. pp. 230-239.
@article{f9007d5024cc43ac95ba06f16da7d9e1,
title = "Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin",
abstract = "Increasing evidence suggests an immunoregulatory function of the potent steroid hormone 1,25-dihydroxyvitamin D3(1,25(OH)2D3) which has been successfully applied for treatment of psoriasis. The skin is both a site of production and a target of 1,25(OH)2D3. In vitro, 1,25(OH)2D3 inhibits proliferation and stimulates differentiation of keratinocytes. We investigated the in situ expression of vitamin D-receptors (VDR) in normal and psoriatic skin by immunochemical methods. The VDR were visualized using the monoclonal antibody (MoAb) 9A7g to the VDR and the labeled avidinbiotin technique. Immunoreactivity was consistently confined to nuclei in all skin biopsies. In normal skin specimens (n = 10) VDR antigens were expressed in keratinocytes of all epidermal layers (except those of the stratum corneum) and in cells of the epidermal appendages. Double labeling experiments with MoAb to cluster-defined antigens indicated that melanocytes and approximately 75{\%} of Langerhans cells exhibit 1,25(OH)2D3 receptors in normal skin biopsies (n = 5). Depending on their localization in skin compartments 42-62{\%} of CD11b+ positive macrophages and 45-75{\%} of CD3+ T lymphocytes expressed VDR. Non-lesional psoriatic skin specimens (n = 8) revealed nearly identical staining patterns. Lesional psoriatic skin specimens (n = 8) exhibited a significant increase of VDR expression both in basal and suprabasal epidermal layers as measured by computer-assisted morphometry and showed a remarkable change of the immune cell pattern: the densitity and proportion of VDR positive T lymphocytes and macrophages were higher in the epidermal and the perivascular papillary loop compartment. These in vivo findings strongly support the hypothesis that 1,25(OH)2D3 modulates immune response and cell proliferation/differentiation in human skin.",
author = "Petra Milde and Ulrike Hauser and Thomas Simon and Gerhard Mall and Volker Ernst and Haussler, {Mark R} and Peter Frosch and Rauterberg, {Ernst W.}",
year = "1991",
month = "8",
language = "English (US)",
volume = "97",
pages = "230--239",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Expression of 1,25-dihydroxyvitamin D3 receptors in normal and psoriatic skin

AU - Milde, Petra

AU - Hauser, Ulrike

AU - Simon, Thomas

AU - Mall, Gerhard

AU - Ernst, Volker

AU - Haussler, Mark R

AU - Frosch, Peter

AU - Rauterberg, Ernst W.

PY - 1991/8

Y1 - 1991/8

N2 - Increasing evidence suggests an immunoregulatory function of the potent steroid hormone 1,25-dihydroxyvitamin D3(1,25(OH)2D3) which has been successfully applied for treatment of psoriasis. The skin is both a site of production and a target of 1,25(OH)2D3. In vitro, 1,25(OH)2D3 inhibits proliferation and stimulates differentiation of keratinocytes. We investigated the in situ expression of vitamin D-receptors (VDR) in normal and psoriatic skin by immunochemical methods. The VDR were visualized using the monoclonal antibody (MoAb) 9A7g to the VDR and the labeled avidinbiotin technique. Immunoreactivity was consistently confined to nuclei in all skin biopsies. In normal skin specimens (n = 10) VDR antigens were expressed in keratinocytes of all epidermal layers (except those of the stratum corneum) and in cells of the epidermal appendages. Double labeling experiments with MoAb to cluster-defined antigens indicated that melanocytes and approximately 75% of Langerhans cells exhibit 1,25(OH)2D3 receptors in normal skin biopsies (n = 5). Depending on their localization in skin compartments 42-62% of CD11b+ positive macrophages and 45-75% of CD3+ T lymphocytes expressed VDR. Non-lesional psoriatic skin specimens (n = 8) revealed nearly identical staining patterns. Lesional psoriatic skin specimens (n = 8) exhibited a significant increase of VDR expression both in basal and suprabasal epidermal layers as measured by computer-assisted morphometry and showed a remarkable change of the immune cell pattern: the densitity and proportion of VDR positive T lymphocytes and macrophages were higher in the epidermal and the perivascular papillary loop compartment. These in vivo findings strongly support the hypothesis that 1,25(OH)2D3 modulates immune response and cell proliferation/differentiation in human skin.

AB - Increasing evidence suggests an immunoregulatory function of the potent steroid hormone 1,25-dihydroxyvitamin D3(1,25(OH)2D3) which has been successfully applied for treatment of psoriasis. The skin is both a site of production and a target of 1,25(OH)2D3. In vitro, 1,25(OH)2D3 inhibits proliferation and stimulates differentiation of keratinocytes. We investigated the in situ expression of vitamin D-receptors (VDR) in normal and psoriatic skin by immunochemical methods. The VDR were visualized using the monoclonal antibody (MoAb) 9A7g to the VDR and the labeled avidinbiotin technique. Immunoreactivity was consistently confined to nuclei in all skin biopsies. In normal skin specimens (n = 10) VDR antigens were expressed in keratinocytes of all epidermal layers (except those of the stratum corneum) and in cells of the epidermal appendages. Double labeling experiments with MoAb to cluster-defined antigens indicated that melanocytes and approximately 75% of Langerhans cells exhibit 1,25(OH)2D3 receptors in normal skin biopsies (n = 5). Depending on their localization in skin compartments 42-62% of CD11b+ positive macrophages and 45-75% of CD3+ T lymphocytes expressed VDR. Non-lesional psoriatic skin specimens (n = 8) revealed nearly identical staining patterns. Lesional psoriatic skin specimens (n = 8) exhibited a significant increase of VDR expression both in basal and suprabasal epidermal layers as measured by computer-assisted morphometry and showed a remarkable change of the immune cell pattern: the densitity and proportion of VDR positive T lymphocytes and macrophages were higher in the epidermal and the perivascular papillary loop compartment. These in vivo findings strongly support the hypothesis that 1,25(OH)2D3 modulates immune response and cell proliferation/differentiation in human skin.

UR - http://www.scopus.com/inward/record.url?scp=0025823930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025823930&partnerID=8YFLogxK

M3 - Article

C2 - 1649228

AN - SCOPUS:0025823930

VL - 97

SP - 230

EP - 239

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -