Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

F. G. Rodrigues, M. N. Santos, T. X T De Carvalho, B. C. Rocha, Michael A Riehle, P. F P Pimenta, E. G. Abraham, M. Jacobs-Lorena, C. F. Alves De Brito, L. A. Moreira

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalInsect Molecular Biology
Volume17
Issue number2
DOIs
StatePublished - Apr 2008

Fingerprint

Plasmodium gallinaceum
phospholipase A2
Aedes
Phospholipases A2
Culicidae
genetically modified organisms
Bee Venoms
Proteins
Oocysts
oocysts
midgut
Anopheles gambiae
ookinetes
promoter regions
DNA
Enzymes
venoms
Malaria
protein structure
Ochlerotatus fluviatilis

Keywords

  • Bee venom phospholipase A
  • Fitness
  • Malaria
  • piggyBac
  • Transformation

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. / Rodrigues, F. G.; Santos, M. N.; De Carvalho, T. X T; Rocha, B. C.; Riehle, Michael A; Pimenta, P. F P; Abraham, E. G.; Jacobs-Lorena, M.; Alves De Brito, C. F.; Moreira, L. A.

In: Insect Molecular Biology, Vol. 17, No. 2, 04.2008, p. 175-183.

Research output: Contribution to journalArticle

Rodrigues, FG, Santos, MN, De Carvalho, TXT, Rocha, BC, Riehle, MA, Pimenta, PFP, Abraham, EG, Jacobs-Lorena, M, Alves De Brito, CF & Moreira, LA 2008, 'Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development', Insect Molecular Biology, vol. 17, no. 2, pp. 175-183. https://doi.org/10.1111/j.1365-2583.2008.00791.x
Rodrigues, F. G. ; Santos, M. N. ; De Carvalho, T. X T ; Rocha, B. C. ; Riehle, Michael A ; Pimenta, P. F P ; Abraham, E. G. ; Jacobs-Lorena, M. ; Alves De Brito, C. F. ; Moreira, L. A. / Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. In: Insect Molecular Biology. 2008 ; Vol. 17, No. 2. pp. 175-183.
@article{2aae95fbf28341578911742972dda6f4,
title = "Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development",
abstract = "The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68{\%} fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.",
keywords = "Bee venom phospholipase A, Fitness, Malaria, piggyBac, Transformation",
author = "Rodrigues, {F. G.} and Santos, {M. N.} and {De Carvalho}, {T. X T} and Rocha, {B. C.} and Riehle, {Michael A} and Pimenta, {P. F P} and Abraham, {E. G.} and M. Jacobs-Lorena and {Alves De Brito}, {C. F.} and Moreira, {L. A.}",
year = "2008",
month = "4",
doi = "10.1111/j.1365-2583.2008.00791.x",
language = "English (US)",
volume = "17",
pages = "175--183",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

AU - Rodrigues, F. G.

AU - Santos, M. N.

AU - De Carvalho, T. X T

AU - Rocha, B. C.

AU - Riehle, Michael A

AU - Pimenta, P. F P

AU - Abraham, E. G.

AU - Jacobs-Lorena, M.

AU - Alves De Brito, C. F.

AU - Moreira, L. A.

PY - 2008/4

Y1 - 2008/4

N2 - The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.

AB - The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.

KW - Bee venom phospholipase A

KW - Fitness

KW - Malaria

KW - piggyBac

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=40849136302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40849136302&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2583.2008.00791.x

DO - 10.1111/j.1365-2583.2008.00791.x

M3 - Article

C2 - 18353106

AN - SCOPUS:40849136302

VL - 17

SP - 175

EP - 183

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 2

ER -