Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene

Xin Chun Chen, Wei Long Liu, Gui Lin Yang, Yong Jun Liu, Xiu Yun Zhu, Hong Mei Zhang, Bo Ping Zhou, Lonnie Lybarger

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope. METHODS: An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT. RESULTS: HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay. CONCLUSION: A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.

Original languageEnglish (US)
Pages (from-to)161-164
Number of pages4
JournalZhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
Volume23
Issue number3
StatePublished - Jun 2009
Externally publishedYes

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Hepatitis B Surface Antigens
Green Fluorescent Proteins
Adenoviridae
Epitopes
Genes
Product Packaging
Oligonucleotides
Digestion
Western Blotting
DNA
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene. / Chen, Xin Chun; Liu, Wei Long; Yang, Gui Lin; Liu, Yong Jun; Zhu, Xiu Yun; Zhang, Hong Mei; Zhou, Bo Ping; Lybarger, Lonnie.

In: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, Vol. 23, No. 3, 06.2009, p. 161-164.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVE: To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope. METHODS: An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT. RESULTS: HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay. CONCLUSION: A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.",
author = "Chen, {Xin Chun} and Liu, {Wei Long} and Yang, {Gui Lin} and Liu, {Yong Jun} and Zhu, {Xiu Yun} and Zhang, {Hong Mei} and Zhou, {Bo Ping} and Lonnie Lybarger",
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T1 - Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene

AU - Chen, Xin Chun

AU - Liu, Wei Long

AU - Yang, Gui Lin

AU - Liu, Yong Jun

AU - Zhu, Xiu Yun

AU - Zhang, Hong Mei

AU - Zhou, Bo Ping

AU - Lybarger, Lonnie

PY - 2009/6

Y1 - 2009/6

N2 - OBJECTIVE: To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope. METHODS: An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT. RESULTS: HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay. CONCLUSION: A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.

AB - OBJECTIVE: To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope. METHODS: An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT. RESULTS: HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay. CONCLUSION: A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.

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