Expression of chorionic gonadotropin α- and β-genes in normal and neoplastic human tissues: Relationship to deoxyribonucleic acid structure

G. KERR Whitfield, Ione A. Kourides

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We have investigated whether the expression of hCG genes can be attributed to changes in the structure of the α- and βhCG genes, such as rearrangements, duplications, or methylation patterns. Various tissues and cell lines were studied: two term placentae, three trophoblastic tumor cell lines, two tumor cell lines ectopically producing α-subunit, normal cells not producing hCG or subunits, and a nonproducing malignancy. Gene structure was explored by restriction enzyme analysis and Southern blotting of DNA, using as probes 32P-labeled plasmids containing α- and βhCG cDNAs. Similarly, methylation was evaluated using the restriction enzymes Msp I, Hpa II, and Hha I, each sensitive to a different pattern of cytosine methylation. No structural changes were observed in α- and βhCG genes, although certain polymorphisms were observed. Analysis of methylation patterns revealed variation of the methylated cytosines; however, no clear correlation was seen between overall methylation or a specific pattern of methylation of these genes and their expression. Although specific methylated nucleotides of regulatory importance may not have been detected by our methods, we can still conclude that neither DNA structural alterations nor patterns of cytosine methylation appear to be major determinants of hCG expression.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalEndocrinology
Volume117
Issue number1
DOIs
StatePublished - Jul 1 1985

ASJC Scopus subject areas

  • Endocrinology

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