Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera

D. J. Fairley, G. Wang, C. Rensing, I. L. Pepper, M. J. Larkin

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Gentisate-1,2-dioxygenase genes (gdoA), with homology to a number of bacterial dioxygenases, and genes encoding a putative coenzyme A (CoA)-synthetase subunit (acdB) and a CoA-thioesterase (tieA) were identified in two haloarchaeal isolates. In Haloarcula sp. D1, gdoA was expressed during growth on 4-hydroxybenzoate but not benzoate, and acdB and tieA were not expressed during growth on any of the aromatic substrates tested. In contrast, gdoA was expressed in Haloferax sp. D1227 during growth on benzoate, 3-hydroxybenzoate, cinnamate and phenylpropionate, and both acdB and tieA were expressed during growth on benzoate, cinnamate and phenylpropionate, but not on 3-hydroxybenzoate. This pattern of induction is consistent with these genes encoding steps in a CoA-mediated benzoate pathway in this strain.

Original languageEnglish (US)
Pages (from-to)691-695
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume73
Issue number3
DOIs
StatePublished - Dec 1 2006

Keywords

  • 3-Hydroxybenzoate
  • Aromatic
  • Benzoate
  • Catabolism
  • Haloarchaea
  • Haloferax

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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