Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera

D. J. Fairley, G. Wang, C. Rensing, Ian L Pepper, M. J. Larkin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Gentisate-1,2-dioxygenase genes (gdoA), with homology to a number of bacterial dioxygenases, and genes encoding a putative coenzyme A (CoA)-synthetase subunit (acdB) and a CoA-thioesterase (tieA) were identified in two haloarchaeal isolates. In Haloarcula sp. D1, gdoA was expressed during growth on 4-hydroxybenzoate but not benzoate, and acdB and tieA were not expressed during growth on any of the aromatic substrates tested. In contrast, gdoA was expressed in Haloferax sp. D1227 during growth on benzoate, 3-hydroxybenzoate, cinnamate and phenylpropionate, and both acdB and tieA were expressed during growth on benzoate, cinnamate and phenylpropionate, but not on 3-hydroxybenzoate. This pattern of induction is consistent with these genes encoding steps in a CoA-mediated benzoate pathway in this strain.

Original languageEnglish (US)
Pages (from-to)691-695
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume73
Issue number3
DOIs
StatePublished - Dec 2006

Fingerprint

gentisate 1,2-dioxygenase
Benzoates
Coenzymes
Phenylpropionates
Genes
Cinnamates
Hydroxybenzoates
Degradation
Gene encoding
Coenzyme A
Growth
Haloferax
Haloarcula
Coenzyme A Ligases
Bacterial Genes
Dioxygenases
Substrates

Keywords

  • 3-Hydroxybenzoate
  • Aromatic
  • Benzoate
  • Catabolism
  • Haloarchaea
  • Haloferax

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera. / Fairley, D. J.; Wang, G.; Rensing, C.; Pepper, Ian L; Larkin, M. J.

In: Applied Microbiology and Biotechnology, Vol. 73, No. 3, 12.2006, p. 691-695.

Research output: Contribution to journalArticle

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AU - Wang, G.

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AU - Pepper, Ian L

AU - Larkin, M. J.

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