Expression of human IgE-binding factor (sCD23) in Escherichia coli

V. A. Kaigorodov, L. R. Ptitsyn, Vadim I Pivniouk, I. A. Kondrat'eva, I. B. Al'tman, R. G. Vasilov

Research output: Contribution to journalArticle

Abstract

Previously, we cloned cDNA coding for type II Fc receptor for human IgE (CD23 or FcERII). A fragment of this cDNA coding for soluble IgE-binding factor fused with a fragment of human interleukin-3 gene was cloned in pBT-IL-3-sCD23 plasmid. It is demonstrated that E. coli strain JM109 (pBT-IL-3-sCD23) expresses the hybrid protein with a yield of 10-15% total cell protein. The recombinant product is represented by equal amounts of two polypeptides with molecular weights of about 24 and 32 kD. Immunological analysis and determination of the amino acid sequence of the N-terminal ends of these polypeptides show that a protein with a molecular weight of 24 kD results from proteolysis of the full-size (32 kD) hybrid protein. The preparation obtained can be used for the development of test kits for CD23.

Original languageEnglish (US)
Pages (from-to)624-628
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume121
Issue number6
StatePublished - Jun 1996
Externally publishedYes

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Escherichia coli
Interleukin-3
Protein Sequence Analysis
Proteins
Complementary DNA
Molecular Weight
Molecular weight
Proteolysis
Peptides
Fc Receptors
Immunoglobulin E
Plasmids
Genes
Amino Acids
immunoglobulin-binding factors

Keywords

  • Expression in E. coli
  • Hybrid protein IL-3-sCD23
  • Low-affinity Fc receptor of human IgE (CD23)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kaigorodov, V. A., Ptitsyn, L. R., Pivniouk, V. I., Kondrat'eva, I. A., Al'tman, I. B., & Vasilov, R. G. (1996). Expression of human IgE-binding factor (sCD23) in Escherichia coli. Bulletin of Experimental Biology and Medicine, 121(6), 624-628.

Expression of human IgE-binding factor (sCD23) in Escherichia coli. / Kaigorodov, V. A.; Ptitsyn, L. R.; Pivniouk, Vadim I; Kondrat'eva, I. A.; Al'tman, I. B.; Vasilov, R. G.

In: Bulletin of Experimental Biology and Medicine, Vol. 121, No. 6, 06.1996, p. 624-628.

Research output: Contribution to journalArticle

Kaigorodov, VA, Ptitsyn, LR, Pivniouk, VI, Kondrat'eva, IA, Al'tman, IB & Vasilov, RG 1996, 'Expression of human IgE-binding factor (sCD23) in Escherichia coli', Bulletin of Experimental Biology and Medicine, vol. 121, no. 6, pp. 624-628.
Kaigorodov VA, Ptitsyn LR, Pivniouk VI, Kondrat'eva IA, Al'tman IB, Vasilov RG. Expression of human IgE-binding factor (sCD23) in Escherichia coli. Bulletin of Experimental Biology and Medicine. 1996 Jun;121(6):624-628.
Kaigorodov, V. A. ; Ptitsyn, L. R. ; Pivniouk, Vadim I ; Kondrat'eva, I. A. ; Al'tman, I. B. ; Vasilov, R. G. / Expression of human IgE-binding factor (sCD23) in Escherichia coli. In: Bulletin of Experimental Biology and Medicine. 1996 ; Vol. 121, No. 6. pp. 624-628.
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