Expression of IL-2 and IL-4 in T Lymphocytes Transformed by Herpesvirus Saimiri

Chin Sheng Chou, Maria M. Medveczky, Peter Geck, Donata Vercelli, Peter G. Medveczky

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize T lymphocytes and cause tumors in rabbits and New World monkeys T cells infected with strain 484-77 of group C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA which encodes open reading frames ORF-1 and ORF-2. ORF-1 encodes a collagen-like oncoprotein. Deletion mutation analysis showed that ORF-1 and ORF-2 are essential for IL-2 independent growth of human T cells infected with H. saimiri. An earlier study also demonstrated that H saimiri-immortalized cells carry functional IL-2 receptors. The work presented in this report investigated whether IL-2 and IL-4 is produced by H. saimiriimmortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 was suggested by cyclosporin A inhibition. IL-4 secretion by monkey T cell cultures was demonstrated by a bioassay and inhibition of bioactivity by an antibody to IL4. The data also show that recombinant IL-4 stimulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expressed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL-4 receptor did not curtail growth of transformed cells. T cells infected with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expression of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiriimmortalized monkey and human T lymphocytes proliferate through autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequences of the virus are involved in expression of lymphokines.

Original languageEnglish (US)
Pages (from-to)418-426
Number of pages9
JournalVirology
Volume208
Issue number2
DOIs
StatePublished - Apr 20 1995
Externally publishedYes

Fingerprint

Saimiriine Herpesvirus 2
Interleukin-4
Open Reading Frames
Interleukin-2
T-Lymphocytes
Interleukin-4 Receptors
Messenger RNA
Haplorhini
Platyrrhini
Saimiri
Antibodies
Lymphokines
Sequence Deletion
Interleukin-2 Receptors
Herpesviridae
Oncogene Proteins
Growth
Biological Assay

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Expression of IL-2 and IL-4 in T Lymphocytes Transformed by Herpesvirus Saimiri. / Chou, Chin Sheng; Medveczky, Maria M.; Geck, Peter; Vercelli, Donata; Medveczky, Peter G.

In: Virology, Vol. 208, No. 2, 20.04.1995, p. 418-426.

Research output: Contribution to journalArticle

Chou, Chin Sheng ; Medveczky, Maria M. ; Geck, Peter ; Vercelli, Donata ; Medveczky, Peter G. / Expression of IL-2 and IL-4 in T Lymphocytes Transformed by Herpesvirus Saimiri. In: Virology. 1995 ; Vol. 208, No. 2. pp. 418-426.
@article{42069e2dd72745a8aa572364b946ee63,
title = "Expression of IL-2 and IL-4 in T Lymphocytes Transformed by Herpesvirus Saimiri",
abstract = "Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize T lymphocytes and cause tumors in rabbits and New World monkeys T cells infected with strain 484-77 of group C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA which encodes open reading frames ORF-1 and ORF-2. ORF-1 encodes a collagen-like oncoprotein. Deletion mutation analysis showed that ORF-1 and ORF-2 are essential for IL-2 independent growth of human T cells infected with H. saimiri. An earlier study also demonstrated that H saimiri-immortalized cells carry functional IL-2 receptors. The work presented in this report investigated whether IL-2 and IL-4 is produced by H. saimiriimmortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 was suggested by cyclosporin A inhibition. IL-4 secretion by monkey T cell cultures was demonstrated by a bioassay and inhibition of bioactivity by an antibody to IL4. The data also show that recombinant IL-4 stimulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expressed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL-4 receptor did not curtail growth of transformed cells. T cells infected with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expression of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiriimmortalized monkey and human T lymphocytes proliferate through autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequences of the virus are involved in expression of lymphokines.",
author = "Chou, {Chin Sheng} and Medveczky, {Maria M.} and Peter Geck and Donata Vercelli and Medveczky, {Peter G.}",
year = "1995",
month = "4",
day = "20",
doi = "10.1006/viro.1995.1172",
language = "English (US)",
volume = "208",
pages = "418--426",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of IL-2 and IL-4 in T Lymphocytes Transformed by Herpesvirus Saimiri

AU - Chou, Chin Sheng

AU - Medveczky, Maria M.

AU - Geck, Peter

AU - Vercelli, Donata

AU - Medveczky, Peter G.

PY - 1995/4/20

Y1 - 1995/4/20

N2 - Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize T lymphocytes and cause tumors in rabbits and New World monkeys T cells infected with strain 484-77 of group C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA which encodes open reading frames ORF-1 and ORF-2. ORF-1 encodes a collagen-like oncoprotein. Deletion mutation analysis showed that ORF-1 and ORF-2 are essential for IL-2 independent growth of human T cells infected with H. saimiri. An earlier study also demonstrated that H saimiri-immortalized cells carry functional IL-2 receptors. The work presented in this report investigated whether IL-2 and IL-4 is produced by H. saimiriimmortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 was suggested by cyclosporin A inhibition. IL-4 secretion by monkey T cell cultures was demonstrated by a bioassay and inhibition of bioactivity by an antibody to IL4. The data also show that recombinant IL-4 stimulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expressed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL-4 receptor did not curtail growth of transformed cells. T cells infected with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expression of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiriimmortalized monkey and human T lymphocytes proliferate through autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequences of the virus are involved in expression of lymphokines.

AB - Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic herpesvirus which can immortalize T lymphocytes and cause tumors in rabbits and New World monkeys T cells infected with strain 484-77 of group C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA which encodes open reading frames ORF-1 and ORF-2. ORF-1 encodes a collagen-like oncoprotein. Deletion mutation analysis showed that ORF-1 and ORF-2 are essential for IL-2 independent growth of human T cells infected with H. saimiri. An earlier study also demonstrated that H saimiri-immortalized cells carry functional IL-2 receptors. The work presented in this report investigated whether IL-2 and IL-4 is produced by H. saimiriimmortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA were detected in various monkey T cells as well as human peripheral blood lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 was suggested by cyclosporin A inhibition. IL-4 secretion by monkey T cell cultures was demonstrated by a bioassay and inhibition of bioactivity by an antibody to IL4. The data also show that recombinant IL-4 stimulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expressed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL-4 receptor did not curtail growth of transformed cells. T cells infected with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expression of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiriimmortalized monkey and human T lymphocytes proliferate through autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequences of the virus are involved in expression of lymphokines.

UR - http://www.scopus.com/inward/record.url?scp=0029008756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029008756&partnerID=8YFLogxK

U2 - 10.1006/viro.1995.1172

DO - 10.1006/viro.1995.1172

M3 - Article

C2 - 7747414

AN - SCOPUS:0029008756

VL - 208

SP - 418

EP - 426

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -