Expression of metalloproteinase genes in human prostate cancer

M. Siadat Pajouh, Raymond B Nagle, R. Breathnach, J. S. Finch, Michael K. Brawer, G. Tim Bowden

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Abstract

Twenty-five surgical specimens of malignant human prostate, 3 lymph nodes with metastatic prostate carcinoma, 11 normal human prostates, as well as 3 human prostate cell lines (DU-145, PC3 and LNCaP) were examined for the expression of the human matrix metalloproteinase-7 gene (MMP-7) from the human collagenase family (originally called PUMP-1 for putative metalloproteinase-1) [Quantin et al. (1989) Biochemistry 28:5327-5334; Muller et al. (1988) Biochem J 253:187-192; Matrisian and Bowden (1990) Semin Cancer Biol 1:107-115]. Northern blots were prepared using total RNA extracted from 18 prostate adenocarcinomas, 2 lymph nodes with metastatic prostate carcinoma and 11 normal human prostates. When the northern blots were hybridized with a32P-labeled MMP-7 cDNA probe, a 1.2-kb mRNA was detected in 14 out of 18 prostate adenocarcinomas, 1 out of 2 metastatic lymph nodes, and 3 out of 11 normal prostates. The 3 human prostate cell lines did not show any evidence of the MMP-7 transcript. In situ hybridization was conducted to localize the MMP-7 mRNA to individual cells using a35S-labeled MMP-7 cRNA. In situ hybridization was carried out on snap-frozen tissue sections of 7 prostate adenocarcinomas and 3 lymph nodes containing metastatic prostate adenocarcinoma using the same tissues previously probed by northern analysis as well as new samples. In situ hybridization revealed that the MMP-7 gene was expressed in the epithelial cells of primary prostate adenocarcinoma as well as in invasive and metastatic cells. MMP-7 expression was also seen focally in some dysplastic glands but not in stroma. Additional northern blot analysis was performed using probes to human type-IV collagenase, type-I collagenase and stromelysin I in human prostate adenocarcinoma as well as normal prostate tissue. Our results indicated that 6 out of 10 adenocarcinoma samples and none of the 4 normal samples were positive for type-IV collagenase transcripts. Tissue samples were also examined for the expression of type-I collagenase (9 adenocarcinomas and 4 normal) and stromelysin I (13 adenocarcinomas) by northern analysis. None of the tissues was found to express the transcripts of interest at detectable levels. These data suggest that certain metalloproteinases are present in prostatic adenocarcinoma and may play a role in invasion and metastasis.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume117
Issue number2
DOIs
StatePublished - Mar 1991

Fingerprint

Metalloproteases
Prostate
Prostatic Neoplasms
Matrix Metalloproteinase 7
Gene Expression
Adenocarcinoma
Collagenases
Genes
Lymph Nodes
Northern Blotting
In Situ Hybridization
Matrix Metalloproteinase 3
Carcinoma
Cell Line
Complementary RNA
Messenger RNA
Frozen Sections
Biochemistry

Keywords

  • Collagenase
  • Matrix metalloproteinase-7 (MMP-7)
  • Metalloproteinase
  • Prostate adenocarcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Expression of metalloproteinase genes in human prostate cancer. / Pajouh, M. Siadat; Nagle, Raymond B; Breathnach, R.; Finch, J. S.; Brawer, Michael K.; Bowden, G. Tim.

In: Journal of Cancer Research and Clinical Oncology, Vol. 117, No. 2, 03.1991, p. 144-150.

Research output: Contribution to journalArticle

Pajouh, MS, Nagle, RB, Breathnach, R, Finch, JS, Brawer, MK & Bowden, GT 1991, 'Expression of metalloproteinase genes in human prostate cancer', Journal of Cancer Research and Clinical Oncology, vol. 117, no. 2, pp. 144-150. https://doi.org/10.1007/BF01613138
Pajouh, M. Siadat ; Nagle, Raymond B ; Breathnach, R. ; Finch, J. S. ; Brawer, Michael K. ; Bowden, G. Tim. / Expression of metalloproteinase genes in human prostate cancer. In: Journal of Cancer Research and Clinical Oncology. 1991 ; Vol. 117, No. 2. pp. 144-150.
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abstract = "Twenty-five surgical specimens of malignant human prostate, 3 lymph nodes with metastatic prostate carcinoma, 11 normal human prostates, as well as 3 human prostate cell lines (DU-145, PC3 and LNCaP) were examined for the expression of the human matrix metalloproteinase-7 gene (MMP-7) from the human collagenase family (originally called PUMP-1 for putative metalloproteinase-1) [Quantin et al. (1989) Biochemistry 28:5327-5334; Muller et al. (1988) Biochem J 253:187-192; Matrisian and Bowden (1990) Semin Cancer Biol 1:107-115]. Northern blots were prepared using total RNA extracted from 18 prostate adenocarcinomas, 2 lymph nodes with metastatic prostate carcinoma and 11 normal human prostates. When the northern blots were hybridized with a32P-labeled MMP-7 cDNA probe, a 1.2-kb mRNA was detected in 14 out of 18 prostate adenocarcinomas, 1 out of 2 metastatic lymph nodes, and 3 out of 11 normal prostates. The 3 human prostate cell lines did not show any evidence of the MMP-7 transcript. In situ hybridization was conducted to localize the MMP-7 mRNA to individual cells using a35S-labeled MMP-7 cRNA. In situ hybridization was carried out on snap-frozen tissue sections of 7 prostate adenocarcinomas and 3 lymph nodes containing metastatic prostate adenocarcinoma using the same tissues previously probed by northern analysis as well as new samples. In situ hybridization revealed that the MMP-7 gene was expressed in the epithelial cells of primary prostate adenocarcinoma as well as in invasive and metastatic cells. MMP-7 expression was also seen focally in some dysplastic glands but not in stroma. Additional northern blot analysis was performed using probes to human type-IV collagenase, type-I collagenase and stromelysin I in human prostate adenocarcinoma as well as normal prostate tissue. Our results indicated that 6 out of 10 adenocarcinoma samples and none of the 4 normal samples were positive for type-IV collagenase transcripts. Tissue samples were also examined for the expression of type-I collagenase (9 adenocarcinomas and 4 normal) and stromelysin I (13 adenocarcinomas) by northern analysis. None of the tissues was found to express the transcripts of interest at detectable levels. These data suggest that certain metalloproteinases are present in prostatic adenocarcinoma and may play a role in invasion and metastasis.",
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