Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Kristi R. Harkins, Richard A. Jefferson, Tony A. Kavanagh, Michael W. Bevan, David W. Galbraith

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

Original languageEnglish (US)
Pages (from-to)816-820
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • Flow cytometry
  • Gene expression
  • Nicotiana tabacum
  • β-glucuronidase

ASJC Scopus subject areas

  • General

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