Expression of the fast twitch troponin complex, fTnT, fTnl and fTnC, in vascular smooth muscle

Carlos M. Moran, Robert J. Garriock, Melanie K. Miller, Ronald L Heimark, Carol Gregorio, Paul A Krieg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (ITnT, fTnl, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that ITnT, fTnl and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function.

Original languageEnglish (US)
Pages (from-to)652-661
Number of pages10
JournalCell Motility and the Cytoskeleton
Volume65
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Troponin
Vascular Smooth Muscle
Smooth Muscle
Tropomyosin
Troponin T
Troponin I
Thoracic Aorta
Fluorescence Microscopy
In Situ Hybridization
Blood Vessels
Aorta
Skeletal Muscle
Proteins

Keywords

  • Intestinal smooth muscle
  • Troponin
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression of the fast twitch troponin complex, fTnT, fTnl and fTnC, in vascular smooth muscle. / Moran, Carlos M.; Garriock, Robert J.; Miller, Melanie K.; Heimark, Ronald L; Gregorio, Carol; Krieg, Paul A.

In: Cell Motility and the Cytoskeleton, Vol. 65, No. 8, 08.2008, p. 652-661.

Research output: Contribution to journalArticle

@article{241c6316d45240929a5bf87d05652eac,
title = "Expression of the fast twitch troponin complex, fTnT, fTnl and fTnC, in vascular smooth muscle",
abstract = "It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (ITnT, fTnl, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that ITnT, fTnl and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function.",
keywords = "Intestinal smooth muscle, Troponin, Vascular smooth muscle",
author = "Moran, {Carlos M.} and Garriock, {Robert J.} and Miller, {Melanie K.} and Heimark, {Ronald L} and Carol Gregorio and Krieg, {Paul A}",
year = "2008",
month = "8",
doi = "10.1002/cm.20291",
language = "English (US)",
volume = "65",
pages = "652--661",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Expression of the fast twitch troponin complex, fTnT, fTnl and fTnC, in vascular smooth muscle

AU - Moran, Carlos M.

AU - Garriock, Robert J.

AU - Miller, Melanie K.

AU - Heimark, Ronald L

AU - Gregorio, Carol

AU - Krieg, Paul A

PY - 2008/8

Y1 - 2008/8

N2 - It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (ITnT, fTnl, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that ITnT, fTnl and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function.

AB - It is generally believed that proteins of the troponin complex are not expressed in smooth muscle. We have directly assayed for expression of troponin transcripts in mouse vascular smooth muscle and found that troponin sequences normally associated with fast twitch skeletal muscle (ITnT, fTnl, fTnC) were present at significant levels in the thoracic aorta. In situ hybridization experiments demonstrated that ITnT, fTnl and fTnC transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. Protein blot analysis using rat tissue showed that at least two members of the troponin complex, Troponin T and Troponin I, were translated in vascular smooth muscle of the aorta. Finally, immuno-fluorescence microscopy of rat aortic smooth muscle revealed that TnT and TnI are localized in a unique pattern, coincident with the distribution of tropomyosin. It seems likely therefore, that a complete troponin complex is expressed in vascular smooth muscle and is associated with the contractile machinery of the cell. These observations raise the possibility that troponins play a role in regulation of smooth muscle function.

KW - Intestinal smooth muscle

KW - Troponin

KW - Vascular smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=49149121216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49149121216&partnerID=8YFLogxK

U2 - 10.1002/cm.20291

DO - 10.1002/cm.20291

M3 - Article

C2 - 18548613

AN - SCOPUS:49149121216

VL - 65

SP - 652

EP - 661

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 8

ER -