Expression of type IV collagenase correlates with the invasion of human lymphoblastoid cell lines and pathogenesis in SCID mice

Mary J C Hendrix, Elisabeth A. Seftor, Thomas M. Grogan, Richard E B Seftor, Evan M Hersh, Edward A. Boyse, Lance A. Liotta, William Stetler-Stevenson, C. George Ray

Research output: Contribution to journalArticle

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Abstract

An in vitro model, called the Membrane Invasion Culture System (MICS), was used to study the invasive potential of an Epstein-Barr virus (EBV) positive lymphoblastoid cell line (LCL), an EBV-negative Burkitt lymphoma (BL) cell line of American origin and an EBV-positive BL of African origin. MICS measured the ability of these cell lines to invade reconstituted basement membrane-coated filters, which correlated with their tumorigenic and metastatic capabilities in a SCID mouse model. Furthermore, the significantly greater invasive behaviour of the EBV-positive LCL was directly correlated with the cells' ability to express and secrete human type IV collagenase (72 kDa), an important metalloproteinase responsible for the degradation of collagen IV in basement membranes. The data suggest that MICS and the SCID mouse are useful tests of tumorigenicity in lymphoid cells, with measurable effects in both systems related to human type IV collagenase activity. Both models allow further exploration of malignant phenotypes associated with EBV transformation of lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)59-65
Number of pages7
JournalMolecular and Cellular Probes
Volume6
Issue number1
DOIs
StatePublished - 1992

Fingerprint

SCID Mice
Collagenases
Human Herpesvirus 4
Cell Line
Aptitude
Burkitt Lymphoma
Basement Membrane
Membranes
Carcinogenicity Tests
Matrix Metalloproteinase 2
Metalloproteases
Lymphoid Tissue
Collagen
Lymphocytes
Phenotype

Keywords

  • Collagenase IV
  • Epstein-Barr virus
  • invasion
  • lymphoblastoid cells
  • lymphoma
  • SCID mouse

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Expression of type IV collagenase correlates with the invasion of human lymphoblastoid cell lines and pathogenesis in SCID mice. / Hendrix, Mary J C; Seftor, Elisabeth A.; Grogan, Thomas M.; Seftor, Richard E B; Hersh, Evan M; Boyse, Edward A.; Liotta, Lance A.; Stetler-Stevenson, William; Ray, C. George.

In: Molecular and Cellular Probes, Vol. 6, No. 1, 1992, p. 59-65.

Research output: Contribution to journalArticle

Hendrix, MJC, Seftor, EA, Grogan, TM, Seftor, REB, Hersh, EM, Boyse, EA, Liotta, LA, Stetler-Stevenson, W & Ray, CG 1992, 'Expression of type IV collagenase correlates with the invasion of human lymphoblastoid cell lines and pathogenesis in SCID mice', Molecular and Cellular Probes, vol. 6, no. 1, pp. 59-65. https://doi.org/10.1016/0890-8508(92)90072-6
Hendrix, Mary J C ; Seftor, Elisabeth A. ; Grogan, Thomas M. ; Seftor, Richard E B ; Hersh, Evan M ; Boyse, Edward A. ; Liotta, Lance A. ; Stetler-Stevenson, William ; Ray, C. George. / Expression of type IV collagenase correlates with the invasion of human lymphoblastoid cell lines and pathogenesis in SCID mice. In: Molecular and Cellular Probes. 1992 ; Vol. 6, No. 1. pp. 59-65.
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