Expression, regulation and function of Na,K-ATPase in the lens

Nicholas A Delamere, Shigeo Tamiya

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Na,K-ATPase is responsible for maintaining the correct concentrations of sodium and potassium in lens cells. Na,K-ATPase activity is different in the two cell types that make up the lens, epithelial cells and fibers; specific activity in the epithelium is higher than in fibers. In some parts of the fiber mass Na,K-ATPase activity is barely detectable. There is a large body of evidence that suggests Na,K-ATPase-mediated ion transport by the epithelium contributes significantly to the regulation of ionic composition in the entire lens. In some species different Na,K-ATPase isoforms are present in epithelium and fibers but in general, fibers and epithelium express a similar amount of Na,K-ATPase protein. Turnover of Na,K-ATPase by protein synthesis may contribute to preservation of high Na,K-ATPase activity in the epithelium. In ageing lens fibers, oxidation, and glycation may decrease Na,K-ATPase activity. Na,K-ATPase activity in lens fibers and epithelium also may be subject to regulation as the result of protein tyrosine phosphorylation. Moreover, activation of G protein-coupled receptors by agonists such as endothelin-1 elicits changes of Na,K-ATPase activity. The asymmetrical distribution of Na,K-ATPase activity in the epithelium and fibers may contribute to ionic currents that flow in and around the lens. Studies on human cataract and experimental cataract in animals reveal changes of Na,K-ATPase activity but no clear pattern is evident. However, there is a convincing link between abnormal elevation of lens sodium and the opacification of the lens cortex that occurs in age-related human cataract.

Original languageEnglish (US)
Pages (from-to)593-615
Number of pages23
JournalProgress in Retinal and Eye Research
Volume23
Issue number6
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Lenses
Epithelium
Cataract
sodium-translocating ATPase
Sodium
Ion Transport
Endothelin-1
G-Protein-Coupled Receptors
Tyrosine
Potassium
Protein Isoforms
Epithelial Cells
Phosphorylation

ASJC Scopus subject areas

  • Sensory Systems
  • Ophthalmology

Cite this

Expression, regulation and function of Na,K-ATPase in the lens. / Delamere, Nicholas A; Tamiya, Shigeo.

In: Progress in Retinal and Eye Research, Vol. 23, No. 6, 11.2004, p. 593-615.

Research output: Contribution to journalArticle

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