Expressional divergence of insect GOX genes: From specialist to generalist glucose oxidase

Lihong Yang, Xiongya Wang, Sufen Bai, Xin Li, Shaohua Gu, Chen Zhu Wang, Xianchun Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Insect herbivores often secrete glucose oxidase (GOX) onto plants to counteract plant defenses and potential pathogens. Whether generalist herbivores always have significantly higher GOX activities than their specialist counterparts at any comparable stage or conditions and how this is realized remain unknown. To address these two general questions, we subjected larvae of a pair of sister species differed mainly in host range, the generalist Helicoverpa armigera and its specialist counterpart Helicoverpa assulta, to the same sets of stage, protein to digestible carbohydrate (P:C) ratio, allelochemical or host plant treatments for simultaneous analyses of GOX transcripts and activities in their labial glands. GOX activity and transcripts are upregulated concurrently with food ingestion and body growth, downregulated with stopping ingestion and wandering for pupation in both species. The three tested host plants upregulated GOX transcripts, and to a lesser extent, GOX activity in both species. There were significant differences in both GOX transcripts and activity elicited by allelochemicals, but only in GOX transcripts by P:C ratios in both species. GOX activities were higher in H. armigera than H. assulta in all the comparable treatments, but GOX transcripts were significantly higher either in generalists or in specialists, depending on the developmental stages, host plants, P:C ratio and allelochemicals they encounter. These data indicate that the greater GOX activity in generalist herbivores is not achieved by greater transcription rate, but by greater transcript stability, greater translation rate, better enzyme stability and/or their combination.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalJournal of Insect Physiology
Volume100
DOIs
StatePublished - Jul 1 2017

Fingerprint

glucose oxidase
Glucose Oxidase
Insects
insects
Genes
genes
Herbivory
Pheromones
allelochemicals
Helicoverpa assulta
herbivores
host plants
Helicoverpa armigera
Eating
ingestion
enzyme stability
Enzyme Stability
Host Specificity
pupation
lips

Keywords

  • Allelochemicals
  • GOX expression
  • Helicoverpa spp
  • Host plant range
  • P:C ratio
  • Post-transcriptional regulation
  • RNA stability
  • Translation

ASJC Scopus subject areas

  • Physiology
  • Insect Science

Cite this

Expressional divergence of insect GOX genes : From specialist to generalist glucose oxidase. / Yang, Lihong; Wang, Xiongya; Bai, Sufen; Li, Xin; Gu, Shaohua; Wang, Chen Zhu; Li, Xianchun.

In: Journal of Insect Physiology, Vol. 100, 01.07.2017, p. 21-27.

Research output: Contribution to journalArticle

Yang, Lihong ; Wang, Xiongya ; Bai, Sufen ; Li, Xin ; Gu, Shaohua ; Wang, Chen Zhu ; Li, Xianchun. / Expressional divergence of insect GOX genes : From specialist to generalist glucose oxidase. In: Journal of Insect Physiology. 2017 ; Vol. 100. pp. 21-27.
@article{81bee42147c9410d97f66f43e4a450da,
title = "Expressional divergence of insect GOX genes: From specialist to generalist glucose oxidase",
abstract = "Insect herbivores often secrete glucose oxidase (GOX) onto plants to counteract plant defenses and potential pathogens. Whether generalist herbivores always have significantly higher GOX activities than their specialist counterparts at any comparable stage or conditions and how this is realized remain unknown. To address these two general questions, we subjected larvae of a pair of sister species differed mainly in host range, the generalist Helicoverpa armigera and its specialist counterpart Helicoverpa assulta, to the same sets of stage, protein to digestible carbohydrate (P:C) ratio, allelochemical or host plant treatments for simultaneous analyses of GOX transcripts and activities in their labial glands. GOX activity and transcripts are upregulated concurrently with food ingestion and body growth, downregulated with stopping ingestion and wandering for pupation in both species. The three tested host plants upregulated GOX transcripts, and to a lesser extent, GOX activity in both species. There were significant differences in both GOX transcripts and activity elicited by allelochemicals, but only in GOX transcripts by P:C ratios in both species. GOX activities were higher in H. armigera than H. assulta in all the comparable treatments, but GOX transcripts were significantly higher either in generalists or in specialists, depending on the developmental stages, host plants, P:C ratio and allelochemicals they encounter. These data indicate that the greater GOX activity in generalist herbivores is not achieved by greater transcription rate, but by greater transcript stability, greater translation rate, better enzyme stability and/or their combination.",
keywords = "Allelochemicals, GOX expression, Helicoverpa spp, Host plant range, P:C ratio, Post-transcriptional regulation, RNA stability, Translation",
author = "Lihong Yang and Xiongya Wang and Sufen Bai and Xin Li and Shaohua Gu and Wang, {Chen Zhu} and Xianchun Li",
year = "2017",
month = "7",
day = "1",
doi = "10.1016/j.jinsphys.2017.05.003",
language = "English (US)",
volume = "100",
pages = "21--27",
journal = "Journal of Insect Physiology",
issn = "0022-1910",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Expressional divergence of insect GOX genes

T2 - From specialist to generalist glucose oxidase

AU - Yang, Lihong

AU - Wang, Xiongya

AU - Bai, Sufen

AU - Li, Xin

AU - Gu, Shaohua

AU - Wang, Chen Zhu

AU - Li, Xianchun

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Insect herbivores often secrete glucose oxidase (GOX) onto plants to counteract plant defenses and potential pathogens. Whether generalist herbivores always have significantly higher GOX activities than their specialist counterparts at any comparable stage or conditions and how this is realized remain unknown. To address these two general questions, we subjected larvae of a pair of sister species differed mainly in host range, the generalist Helicoverpa armigera and its specialist counterpart Helicoverpa assulta, to the same sets of stage, protein to digestible carbohydrate (P:C) ratio, allelochemical or host plant treatments for simultaneous analyses of GOX transcripts and activities in their labial glands. GOX activity and transcripts are upregulated concurrently with food ingestion and body growth, downregulated with stopping ingestion and wandering for pupation in both species. The three tested host plants upregulated GOX transcripts, and to a lesser extent, GOX activity in both species. There were significant differences in both GOX transcripts and activity elicited by allelochemicals, but only in GOX transcripts by P:C ratios in both species. GOX activities were higher in H. armigera than H. assulta in all the comparable treatments, but GOX transcripts were significantly higher either in generalists or in specialists, depending on the developmental stages, host plants, P:C ratio and allelochemicals they encounter. These data indicate that the greater GOX activity in generalist herbivores is not achieved by greater transcription rate, but by greater transcript stability, greater translation rate, better enzyme stability and/or their combination.

AB - Insect herbivores often secrete glucose oxidase (GOX) onto plants to counteract plant defenses and potential pathogens. Whether generalist herbivores always have significantly higher GOX activities than their specialist counterparts at any comparable stage or conditions and how this is realized remain unknown. To address these two general questions, we subjected larvae of a pair of sister species differed mainly in host range, the generalist Helicoverpa armigera and its specialist counterpart Helicoverpa assulta, to the same sets of stage, protein to digestible carbohydrate (P:C) ratio, allelochemical or host plant treatments for simultaneous analyses of GOX transcripts and activities in their labial glands. GOX activity and transcripts are upregulated concurrently with food ingestion and body growth, downregulated with stopping ingestion and wandering for pupation in both species. The three tested host plants upregulated GOX transcripts, and to a lesser extent, GOX activity in both species. There were significant differences in both GOX transcripts and activity elicited by allelochemicals, but only in GOX transcripts by P:C ratios in both species. GOX activities were higher in H. armigera than H. assulta in all the comparable treatments, but GOX transcripts were significantly higher either in generalists or in specialists, depending on the developmental stages, host plants, P:C ratio and allelochemicals they encounter. These data indicate that the greater GOX activity in generalist herbivores is not achieved by greater transcription rate, but by greater transcript stability, greater translation rate, better enzyme stability and/or their combination.

KW - Allelochemicals

KW - GOX expression

KW - Helicoverpa spp

KW - Host plant range

KW - P:C ratio

KW - Post-transcriptional regulation

KW - RNA stability

KW - Translation

UR - http://www.scopus.com/inward/record.url?scp=85019265653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019265653&partnerID=8YFLogxK

U2 - 10.1016/j.jinsphys.2017.05.003

DO - 10.1016/j.jinsphys.2017.05.003

M3 - Article

C2 - 28512014

AN - SCOPUS:85019265653

VL - 100

SP - 21

EP - 27

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

SN - 0022-1910

ER -