Extraction of serologic and delayed hypersensitivity antigens from spherules of Coccidioides immitis

John N Galgiani, Kris O. Dugger, Neil M. Ampel, Sung H. Sun, John H. Law

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have used an aqueous toluene extraction procedure to obtain antigens from mature spherules of Coccidioides immitis. This extract contained many antigens as determined by immunoblotting and two-dimensional immunoelectrophoretic studies. These included antigens with specificity for tube precipitin-type antibodies having molecular weights ≥ 100 KDa. The extract also displayed lymphocyte-transforming activity when tested on human peripheral blood mononuclear leukocytes from donors who react to coccidioidal skin tests but elicited no such stimulation of cells from persons whose coccidioidal skin tests were nonreactive. At high concentrations of the extract, lymphocyte transformation did not occur, a finding that could not be explained by nonspecific toxicity. When gel filtration was employed to separate antigens by size, tube precipitin-like activity and specific coccidioidal delayed-type hypersensitivity displayed overlap, although only the latter activity was apparent in lower molecular weight pools.

Original languageEnglish (US)
Pages (from-to)65-80
Number of pages16
JournalDiagnostic Microbiology and Infectious Disease
Volume11
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Coccidioides
Delayed Hypersensitivity
Antigens
Precipitins
Skin Tests
Molecular Weight
Mononuclear Leukocytes
Toluene
Lymphocyte Activation
Immunoblotting
Gel Chromatography
Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Extraction of serologic and delayed hypersensitivity antigens from spherules of Coccidioides immitis. / Galgiani, John N; Dugger, Kris O.; Ampel, Neil M.; Sun, Sung H.; Law, John H.

In: Diagnostic Microbiology and Infectious Disease, Vol. 11, No. 2, 1988, p. 65-80.

Research output: Contribution to journalArticle

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