Extracts of electric lobe and electric organ from Torpedo californica increase the total number as well as the number of aggregates of chick myotube acetylcholine receptors

J. A. Connolly, Paul A St John, G. D. Fischbach

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Saline extracts prepared from the electric lobe, the electromotor nerves, and the electric organ (the electromotor system) of Torpedo californica increase the number of ACh receptors on uninnervated chick myotubes in culture, while extracts from T. californica liver or skeletal muscle do not. The extracts also increase the ACh sensitivity of treated myotubes, indicating that newly synthesized receptors are functional. The active substance(s) is heat sensitive but not trypsin sensitive. Gel filtration on Bio-Gel P-150 shows that the activity is associated with a peak of low (<5,000-dalton) molecular weight activity. Labeling studies with rhodamine-conjugated α-bungarotoxin show that, in addition to their effect on receptor number, these extracts also cause aggregation of prelabeled ACh receptors on the myotube surface.

Original languageEnglish (US)
Pages (from-to)1207-1213
Number of pages7
JournalJournal of Neuroscience
Volume2
Issue number9
StatePublished - 1982
Externally publishedYes

Fingerprint

Electric Organ
Torpedo
Skeletal Muscle Fibers
Cholinergic Receptors
Bungarotoxins
Rhodamines
Trypsin
Gel Chromatography
Skeletal Muscle
Hot Temperature
Molecular Weight
Gels
Liver

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Extracts of electric lobe and electric organ from Torpedo californica increase the total number as well as the number of aggregates of chick myotube acetylcholine receptors",
abstract = "Saline extracts prepared from the electric lobe, the electromotor nerves, and the electric organ (the electromotor system) of Torpedo californica increase the number of ACh receptors on uninnervated chick myotubes in culture, while extracts from T. californica liver or skeletal muscle do not. The extracts also increase the ACh sensitivity of treated myotubes, indicating that newly synthesized receptors are functional. The active substance(s) is heat sensitive but not trypsin sensitive. Gel filtration on Bio-Gel P-150 shows that the activity is associated with a peak of low (<5,000-dalton) molecular weight activity. Labeling studies with rhodamine-conjugated α-bungarotoxin show that, in addition to their effect on receptor number, these extracts also cause aggregation of prelabeled ACh receptors on the myotube surface.",
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AU - St John, Paul A

AU - Fischbach, G. D.

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