Abstract
Electrospinning of natural polymer nanofibers useful for biomedical applications often requires the use of cytotoxic organic solvents. In this study, gelatin nanofibers are electrospun from phosphate buffer saline/ethanol binary mixtures as a benign solvent at ambient temperature. The influences of ionic strength, ethanol concentration, and gelatin concentration on the electrospinnability of gelatin solutions and the fiber microarchitectures are analyzed. The electrospun scaffolds retain their morphologies during vapor-phase crosslinking with glutaraldehyde in ethanol and the subsequent removal of salts contained in the nanofibers via water rinsing. When fully hydrated, the mechanically preconditioned scaffolds display a Young's modulus of 25.5 ± 5.3 kPa, tensile strength of 55.5 ± 13.9 kPa, deformability of 160 ± 15%, and resilience of 89.9 ± 1.8%. When cultured on the gelatin scaffolds, 3T3 fibroblasts displayed spindle-like morphology, similar to the cell's normal morphology in a 3D extracellular matrix.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1026-1036 |
| Number of pages | 11 |
| Journal | Biopolymers - Peptide Science Section |
| Volume | 97 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2012 |
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Keywords
- cytobiocompatibility
- electrospinning
- gelatin nanofibers
- mechanical property
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Biomaterials
- Organic Chemistry
Cite this
Fabrication of gelatin nanofibrous scaffolds using ethanol/phosphate buffer saline as a benign solvent. / Zha, Zhengbao; Teng, Weibing; Markle, Valerie; Dai, Zhifei; Wu, Xiaoyi.
In: Biopolymers - Peptide Science Section, Vol. 97, No. 12, 12.2012, p. 1026-1036.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of gelatin nanofibrous scaffolds using ethanol/phosphate buffer saline as a benign solvent
AU - Zha, Zhengbao
AU - Teng, Weibing
AU - Markle, Valerie
AU - Dai, Zhifei
AU - Wu, Xiaoyi
PY - 2012/12
Y1 - 2012/12
N2 - Electrospinning of natural polymer nanofibers useful for biomedical applications often requires the use of cytotoxic organic solvents. In this study, gelatin nanofibers are electrospun from phosphate buffer saline/ethanol binary mixtures as a benign solvent at ambient temperature. The influences of ionic strength, ethanol concentration, and gelatin concentration on the electrospinnability of gelatin solutions and the fiber microarchitectures are analyzed. The electrospun scaffolds retain their morphologies during vapor-phase crosslinking with glutaraldehyde in ethanol and the subsequent removal of salts contained in the nanofibers via water rinsing. When fully hydrated, the mechanically preconditioned scaffolds display a Young's modulus of 25.5 ± 5.3 kPa, tensile strength of 55.5 ± 13.9 kPa, deformability of 160 ± 15%, and resilience of 89.9 ± 1.8%. When cultured on the gelatin scaffolds, 3T3 fibroblasts displayed spindle-like morphology, similar to the cell's normal morphology in a 3D extracellular matrix.
AB - Electrospinning of natural polymer nanofibers useful for biomedical applications often requires the use of cytotoxic organic solvents. In this study, gelatin nanofibers are electrospun from phosphate buffer saline/ethanol binary mixtures as a benign solvent at ambient temperature. The influences of ionic strength, ethanol concentration, and gelatin concentration on the electrospinnability of gelatin solutions and the fiber microarchitectures are analyzed. The electrospun scaffolds retain their morphologies during vapor-phase crosslinking with glutaraldehyde in ethanol and the subsequent removal of salts contained in the nanofibers via water rinsing. When fully hydrated, the mechanically preconditioned scaffolds display a Young's modulus of 25.5 ± 5.3 kPa, tensile strength of 55.5 ± 13.9 kPa, deformability of 160 ± 15%, and resilience of 89.9 ± 1.8%. When cultured on the gelatin scaffolds, 3T3 fibroblasts displayed spindle-like morphology, similar to the cell's normal morphology in a 3D extracellular matrix.
KW - cytobiocompatibility
KW - electrospinning
KW - gelatin nanofibers
KW - mechanical property
UR - http://www.scopus.com/inward/record.url?scp=84866392246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866392246&partnerID=8YFLogxK
U2 - 10.1002/bip.22120
DO - 10.1002/bip.22120
M3 - Article
C2 - 22987593
AN - SCOPUS:84866392246
VL - 97
SP - 1026
EP - 1036
JO - Biopolymers
JF - Biopolymers
SN - 0006-3525
IS - 12
ER -