Fabrication of microchannels with patterned bio-active layers

Ryo Iwama, Lap Man Lee, Eung San Cho, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Patterns of bio-active coatings on the inner surfaces of microchannels have been realized using a novel low-temperature, UV-epoxy glass-to-silicon bonding technique. Sucrose is applied as a protection layer for the immobilized bio-functional films during the bonding step. The bio-functional layer is composed of antibody patterns, for binding specific targets, next to polyethylene glycol (PEG) coated regions for preventing non-specific absorption. The activity of the patterned bio layer is tested, after the removal of the sucrose protection layer, utilizing fluorescent microscopy. A solution of fluorescent-labelled antigens is injected into the microchannels for incubation with the immobilized antibodies. Upon exposure to proper radiation, light is emitted only from the antibody patterns while the PEG regions remain dark. Hence, the sucrose-protection and UV-bonding techniques have not significantly compromised the functionality of the patterned antibodies, in binding to their counter receptors, and PEG molecules, in preventing non-specific adsorption, at the end of the fabrication process.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages333-336
Number of pages4
StatePublished - 2007
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: Jan 21 2007Jan 25 2007

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period1/21/071/25/07

Fingerprint

microchannels
Microchannels
antibodies
Antibodies
Sucrose
sucrose
Sugar (sucrose)
Polyethylene glycols
glycols
polyethylenes
Fabrication
fabrication
Immobilized Antibodies
Silicon
Microscopic examination
antigens
Antigens
Radiation
Adsorption
Glass

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Iwama, R., Lee, L. M., Cho, E. S., & Zohar, Y. (2007). Fabrication of microchannels with patterned bio-active layers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 333-336). [4433066]

Fabrication of microchannels with patterned bio-active layers. / Iwama, Ryo; Lee, Lap Man; Cho, Eung San; Zohar, Yitshak.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 333-336 4433066.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Iwama, R, Lee, LM, Cho, ES & Zohar, Y 2007, Fabrication of microchannels with patterned bio-active layers. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 4433066, pp. 333-336, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 1/21/07.
Iwama R, Lee LM, Cho ES, Zohar Y. Fabrication of microchannels with patterned bio-active layers. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 333-336. 4433066
Iwama, Ryo ; Lee, Lap Man ; Cho, Eung San ; Zohar, Yitshak. / Fabrication of microchannels with patterned bio-active layers. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. pp. 333-336
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