Factors that influence the development of cultured neurons from the brain of the moth Manduca sexta

Lynne A Oland, H. Oberlander

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

During metamorphic adult development, neurons and glial cells in the developing olfactory (antennal) lobes of the moth undergo characteristic and extensive changes in shape. These changes depend on an interplay among these two cell types and ingrowing sensory axons. All of the direct cellular interactions occur against a background of changing steroid hormone titers. Antennal-lobe (AL) neurons dissociated from stage-5 (of 18 stages) metamorphosing animals survive at least 3 wk in primary cell culture. We describe here the morphological influences on AL neurons of (1) exposure to the steroid hormone 20-hydroxyecdysone, (2) exposure to sensory axons, and (3) interactions among the AL neurons. Cultured AL neurons respond only weakly, if at all, to 20-hydroxyecdysone. They do, however, show greater total outgrowth and branching when they had been exposed in vivo to sensory axons. Because there is no direct contact between some of the neuronal types and the sensory axons at the time of dissociation, the increase in outgrowth must have been mediated via a diffusible factor(s). When AL cells (neurons and glia) are plated at high density in low volumes of medium, or when the cells are plated at low density but in the presence of medium conditioned by high-density cultures, neurite outgrowth and cell survival are increased. Nerve growth factor (NGF), epidermal growth factor (EGF), fibroblast growth factor-basic (bFGF), transforming growth factor-β (TGF(β)) and insulin- like growth factor (ILGF) had no obvious effect on neuronal morphology and thus are unlikely to underlie these effects. Our results suggest that the mature shape of AL neurons depends on developmental interactions among a number of diffusible factors.

Original languageEnglish (US)
Pages (from-to)709-716
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume30 A
Issue number10
StatePublished - 1994

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Manduca
Moths
Neurons
Brain
Axons
Steroid hormones
Ecdysterone
Cell culture
Neuroglia
Steroids
Hormones
Primary Cell Culture
Transforming Growth Factors
Nerve Growth Factor
Somatomedins
Fibroblast Growth Factor 2
Conditioned Culture Medium
Epidermal Growth Factor
Cell Survival
Animals

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Factors that influence the development of cultured neurons from the brain of the moth Manduca sexta. / Oland, Lynne A; Oberlander, H.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 30 A, No. 10, 1994, p. 709-716.

Research output: Contribution to journalArticle

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