Fas receptor expression on asthmatic eosinophils

Judith E. Fahy, Stuart F Quan, John W Bloom, Marilyn J. Halonen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Activation of the Fas receptor (Fas; CD95) on eosinophils induces apoptosis and may play a role in controlling eosinophil number in blood and tissue. To determine whether Fas expression on eosinophils is different between asthmatics and nonasthmatics, eosinophils were isolated from the peripheral blood using a three-step procedure of dextran sedimentation, ficoll hypaque separation and negative selection with anti-CD16 monoclonal antibody bound to magnetic beads. Fas expression was quantified by flow cytometry. Fas expression and the percentage of eosinophils expressing Fas were not different between asthmatics (n=9) and non-asthmatics (n=14).

Original languageEnglish (US)
Pages (from-to)93-96
Number of pages4
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume102
Issue number1
StatePublished - 1998

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CD95 Antigens
Eosinophils
Diatrizoate
Ficoll
Dextrans
Flow Cytometry
Monoclonal Antibodies
Apoptosis

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pathology and Forensic Medicine

Cite this

Fas receptor expression on asthmatic eosinophils. / Fahy, Judith E.; Quan, Stuart F; Bloom, John W; Halonen, Marilyn J.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 102, No. 1, 1998, p. 93-96.

Research output: Contribution to journalArticle

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