Fate of wastewater effluent hER-agonists and hER-antagonists during soil aquifer treatment

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Estrogen activity was measured in wastewater effluent before and after polishing via soil-aquifer treatment (SAT) using both a (hER-β) competitive binding assay and a transcriptional activation (yeast estrogen screen, YES) assay. From the competitive binding assay, the equivalent 17α- ethinylestradiol (EE2) concentration in secondary effluent was 4.7 nM but decreased to 0.22 nM following SAT. The YES assay indicated that the equivalent EE2 concentration in the same effluent sample was below the method-detection limit (<2.5 × 10-3 nM) but increased to 0.68 nM in effluent polished via SAT processes. It was hypothesized that test-dependent differences arose because the competitive binding assay responds positively to both estrogen mimics and anti-estrogens; the YES assay responds to estrogen mimics, but test response is inhibited by anti-estrogens. The hypothesis was supported when organics extracted from wastewater effluent inhibited the YES test response to EE2 (anti-estrogenic effect). A similar extract prepared from SAT-polished effluent augmented the EE2 curve (agonist response). When hydrophobic organics in secondary effluent were fractionated, assay results indicated that several physically distinct anti-estrogens were present in the sample. From this work, it is evident that transcription-activation bioassays alone should not be relied upon to measure estrogenic activity in complex environmental samples because the simultaneous presence of both agonists and antagonist compounds can yield false negatives. Multiple in vitro bioassays, sample fractionation or tests designed to measure anti-estrogenic activity can be used to overcome this problem. It is also clear that there are circumstances under which SAT does not completely remove estrogenic activity during municipal wastewater effluent polishing.

Original languageEnglish (US)
Pages (from-to)2287-2293
Number of pages7
JournalEnvironmental Science and Technology
Volume39
Issue number7
DOIs
StatePublished - Apr 1 2005

Fingerprint

Aquifers
Effluents
Estrogens
Wastewater
aquifer
effluent
Soils
wastewater
assay
Assays
yeast
soil
Yeast
bioassay
Bioassay
Polishing
Chemical activation
detection method
Effluent treatment
Ethinyl Estradiol

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Fate of wastewater effluent hER-agonists and hER-antagonists during soil aquifer treatment. / Conroy, Otakuye; Quanrud, David M; Ela, Wendell P; Wicke, Daniel; Lansey, Kevin E; Arnold, Robert G.

In: Environmental Science and Technology, Vol. 39, No. 7, 01.04.2005, p. 2287-2293.

Research output: Contribution to journalArticle

@article{422f3d8a93554a3bad57f36ce3663a50,
title = "Fate of wastewater effluent hER-agonists and hER-antagonists during soil aquifer treatment",
abstract = "Estrogen activity was measured in wastewater effluent before and after polishing via soil-aquifer treatment (SAT) using both a (hER-β) competitive binding assay and a transcriptional activation (yeast estrogen screen, YES) assay. From the competitive binding assay, the equivalent 17α- ethinylestradiol (EE2) concentration in secondary effluent was 4.7 nM but decreased to 0.22 nM following SAT. The YES assay indicated that the equivalent EE2 concentration in the same effluent sample was below the method-detection limit (<2.5 × 10-3 nM) but increased to 0.68 nM in effluent polished via SAT processes. It was hypothesized that test-dependent differences arose because the competitive binding assay responds positively to both estrogen mimics and anti-estrogens; the YES assay responds to estrogen mimics, but test response is inhibited by anti-estrogens. The hypothesis was supported when organics extracted from wastewater effluent inhibited the YES test response to EE2 (anti-estrogenic effect). A similar extract prepared from SAT-polished effluent augmented the EE2 curve (agonist response). When hydrophobic organics in secondary effluent were fractionated, assay results indicated that several physically distinct anti-estrogens were present in the sample. From this work, it is evident that transcription-activation bioassays alone should not be relied upon to measure estrogenic activity in complex environmental samples because the simultaneous presence of both agonists and antagonist compounds can yield false negatives. Multiple in vitro bioassays, sample fractionation or tests designed to measure anti-estrogenic activity can be used to overcome this problem. It is also clear that there are circumstances under which SAT does not completely remove estrogenic activity during municipal wastewater effluent polishing.",
author = "Otakuye Conroy and Quanrud, {David M} and Ela, {Wendell P} and Daniel Wicke and Lansey, {Kevin E} and Arnold, {Robert G}",
year = "2005",
month = "4",
day = "1",
doi = "10.1021/es049490b",
language = "English (US)",
volume = "39",
pages = "2287--2293",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Fate of wastewater effluent hER-agonists and hER-antagonists during soil aquifer treatment

AU - Conroy, Otakuye

AU - Quanrud, David M

AU - Ela, Wendell P

AU - Wicke, Daniel

AU - Lansey, Kevin E

AU - Arnold, Robert G

PY - 2005/4/1

Y1 - 2005/4/1

N2 - Estrogen activity was measured in wastewater effluent before and after polishing via soil-aquifer treatment (SAT) using both a (hER-β) competitive binding assay and a transcriptional activation (yeast estrogen screen, YES) assay. From the competitive binding assay, the equivalent 17α- ethinylestradiol (EE2) concentration in secondary effluent was 4.7 nM but decreased to 0.22 nM following SAT. The YES assay indicated that the equivalent EE2 concentration in the same effluent sample was below the method-detection limit (<2.5 × 10-3 nM) but increased to 0.68 nM in effluent polished via SAT processes. It was hypothesized that test-dependent differences arose because the competitive binding assay responds positively to both estrogen mimics and anti-estrogens; the YES assay responds to estrogen mimics, but test response is inhibited by anti-estrogens. The hypothesis was supported when organics extracted from wastewater effluent inhibited the YES test response to EE2 (anti-estrogenic effect). A similar extract prepared from SAT-polished effluent augmented the EE2 curve (agonist response). When hydrophobic organics in secondary effluent were fractionated, assay results indicated that several physically distinct anti-estrogens were present in the sample. From this work, it is evident that transcription-activation bioassays alone should not be relied upon to measure estrogenic activity in complex environmental samples because the simultaneous presence of both agonists and antagonist compounds can yield false negatives. Multiple in vitro bioassays, sample fractionation or tests designed to measure anti-estrogenic activity can be used to overcome this problem. It is also clear that there are circumstances under which SAT does not completely remove estrogenic activity during municipal wastewater effluent polishing.

AB - Estrogen activity was measured in wastewater effluent before and after polishing via soil-aquifer treatment (SAT) using both a (hER-β) competitive binding assay and a transcriptional activation (yeast estrogen screen, YES) assay. From the competitive binding assay, the equivalent 17α- ethinylestradiol (EE2) concentration in secondary effluent was 4.7 nM but decreased to 0.22 nM following SAT. The YES assay indicated that the equivalent EE2 concentration in the same effluent sample was below the method-detection limit (<2.5 × 10-3 nM) but increased to 0.68 nM in effluent polished via SAT processes. It was hypothesized that test-dependent differences arose because the competitive binding assay responds positively to both estrogen mimics and anti-estrogens; the YES assay responds to estrogen mimics, but test response is inhibited by anti-estrogens. The hypothesis was supported when organics extracted from wastewater effluent inhibited the YES test response to EE2 (anti-estrogenic effect). A similar extract prepared from SAT-polished effluent augmented the EE2 curve (agonist response). When hydrophobic organics in secondary effluent were fractionated, assay results indicated that several physically distinct anti-estrogens were present in the sample. From this work, it is evident that transcription-activation bioassays alone should not be relied upon to measure estrogenic activity in complex environmental samples because the simultaneous presence of both agonists and antagonist compounds can yield false negatives. Multiple in vitro bioassays, sample fractionation or tests designed to measure anti-estrogenic activity can be used to overcome this problem. It is also clear that there are circumstances under which SAT does not completely remove estrogenic activity during municipal wastewater effluent polishing.

UR - http://www.scopus.com/inward/record.url?scp=16844367743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16844367743&partnerID=8YFLogxK

U2 - 10.1021/es049490b

DO - 10.1021/es049490b

M3 - Article

VL - 39

SP - 2287

EP - 2293

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 7

ER -